Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 890023..890680 Replicon chromosome
Accession NZ_CP091470
Organism Klebsiella michiganensis strain KO_408

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag L3249_RS04330 Protein ID WP_004854060.1
Coordinates 890270..890680 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag L3249_RS04325 Protein ID WP_004124953.1
Coordinates 890023..890289 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3249_RS04310 (L3249_04325) 885257..885682 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
L3249_RS04315 (L3249_04330) 885803..888601 - 2799 WP_195377494.1 transcriptional regulator DagR -
L3249_RS04320 (L3249_04335) 888795..889778 - 984 WP_025105971.1 tRNA-modifying protein YgfZ -
L3249_RS04325 (L3249_04340) 890023..890289 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
L3249_RS04330 (L3249_04345) 890270..890680 + 411 WP_004854060.1 protein YgfX Toxin
L3249_RS04335 (L3249_04350) 890689..891210 - 522 WP_014226792.1 flavodoxin FldB -
L3249_RS04340 (L3249_04355) 891332..892228 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
L3249_RS04345 (L3249_04360) 892251..892964 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L3249_RS04350 (L3249_04365) 892970..894703 + 1734 WP_032693806.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T233360 WP_004854060.1 NZ_CP091470:890270-890680 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T233360 NZ_HG813240:c2181278-2180949 [Mycobacterium tuberculosis 49-02]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT233360 WP_004124953.1 NZ_CP091470:890023-890289 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT233360 NZ_HG813240:c2181652-2181275 [Mycobacterium tuberculosis 49-02]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References