Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65500..66047 Replicon chromosome
Accession NZ_CP091470
Organism Klebsiella michiganensis strain KO_408

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag L3249_RS00325 Protein ID WP_014227299.1
Coordinates 65500..65808 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag L3249_RS00330 Protein ID WP_014227298.1
Coordinates 65811..66047 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3249_RS00295 (L3249_00295) 61147..61458 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
L3249_RS00300 (L3249_00300) 61753..62694 + 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
L3249_RS00305 (L3249_00305) 62718..63011 - 294 WP_014227302.1 YicS family protein -
L3249_RS00310 (L3249_00310) 63221..64186 + 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
L3249_RS00315 (L3249_00315) 64186..64443 + 258 WP_032718834.1 hypothetical protein -
L3249_RS00320 (L3249_00320) 64447..65349 - 903 WP_014227300.1 EamA family transporter -
L3249_RS00325 (L3249_00325) 65500..65808 - 309 WP_014227299.1 CcdB family protein Toxin
L3249_RS00330 (L3249_00330) 65811..66047 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
L3249_RS00335 (L3249_00335) 66153..67586 - 1434 WP_032718833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
L3249_RS00340 (L3249_00340) 67611..68513 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
L3249_RS00345 (L3249_00345) 68674..69840 - 1167 WP_195378383.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T233359 WP_014227299.1 NZ_CP091470:c65808-65500 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T233359 NZ_HG813240:1962115-1962363 [Mycobacterium tuberculosis 49-02]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT233359 WP_014227298.1 NZ_CP091470:c66047-65811 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT233359 NZ_HG813240:1961903-1962115 [Mycobacterium tuberculosis 49-02]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References