Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 2855989..2856622 Replicon chromosome
Accession NZ_CP091468
Organism Weizmannia coagulans strain 19-1-JSRL-34-1

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag L3V65_RS14015 Protein ID WP_013860680.1
Coordinates 2855989..2856339 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag L3V65_RS14020 Protein ID WP_014096808.1
Coordinates 2856344..2856622 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3V65_RS13980 (L3V65_13980) 2851177..2851955 - 779 Protein_2685 RNA polymerase sigma factor SigB -
L3V65_RS13985 (L3V65_13985) 2851933..2852403 - 471 WP_017553287.1 anti-sigma B factor RsbW -
L3V65_RS13990 (L3V65_13990) 2852407..2852736 - 330 WP_013860674.1 anti-sigma factor antagonist -
L3V65_RS13995 (L3V65_13995) 2852805..2853815 - 1011 WP_237348660.1 PP2C family protein-serine/threonine phosphatase -
L3V65_RS14000 (L3V65_14000) 2853830..2854231 - 402 WP_237348661.1 anti-sigma regulatory factor -
L3V65_RS14005 (L3V65_14005) 2854236..2854592 - 357 WP_133536144.1 STAS domain-containing protein -
L3V65_RS14010 (L3V65_14010) 2854596..2855423 - 828 WP_237348662.1 RsbT co-antagonist protein RsbRA -
L3V65_RS14015 (L3V65_14015) 2855989..2856339 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L3V65_RS14020 (L3V65_14020) 2856344..2856622 - 279 WP_014096808.1 hypothetical protein Antitoxin
L3V65_RS14025 (L3V65_14025) 2856775..2857926 - 1152 WP_237348663.1 alanine racemase -
L3V65_RS14030 (L3V65_14030) 2858128..2859144 - 1017 WP_061574533.1 outer membrane lipoprotein carrier protein LolA -
L3V65_RS14035 (L3V65_14035) 2859285..2859635 - 351 WP_029142790.1 holo-ACP synthase -
L3V65_RS14040 (L3V65_14040) 2859917..2860516 + 600 WP_237348664.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T233357 WP_013860680.1 NZ_CP091468:c2856339-2855989 [Weizmannia coagulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T233357 NZ_HG813240:1768245-1768649 [Mycobacterium tuberculosis 49-02]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT233357 WP_014096808.1 NZ_CP091468:c2856622-2856344 [Weizmannia coagulans]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT233357 NZ_HG813240:1768036-1768239 [Mycobacterium tuberculosis 49-02]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References