Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 122945..123588 Replicon plasmid pMHW-2
Accession NZ_CP091428
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag L3Z43_RS24015 Protein ID WP_001034044.1
Coordinates 123172..123588 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag L3Z43_RS24010 Protein ID WP_001261286.1
Coordinates 122945..123175 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS23990 (119576) 119576..120331 - 756 WP_000852146.1 replication initiation protein RepE -
L3Z43_RS23995 (121053) 121053..121859 - 807 WP_000016970.1 site-specific integrase -
L3Z43_RS24000 (121860) 121860..122165 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
L3Z43_RS24005 (122167) 122167..122385 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
L3Z43_RS24010 (122945) 122945..123175 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L3Z43_RS24015 (123172) 123172..123588 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
L3Z43_RS24020 (123663) 123663..125228 + 1566 WP_001128474.1 AAA family ATPase -
L3Z43_RS24025 (125213) 125213..126235 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..145445 145445
- flank IS/Tn - - 126489..126992 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T233335 WP_001034044.1 NZ_CP091428:123172-123588 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T233335 NZ_HG813240:c443150-442557 [Mycobacterium tuberculosis 49-02]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT233335 WP_001261286.1 NZ_CP091428:122945-123175 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT233335 NZ_HG813240:c443568-443179 [Mycobacterium tuberculosis 49-02]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References