Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 121860..122385 Replicon plasmid pMHW-2
Accession NZ_CP091428
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag L3Z43_RS24000 Protein ID WP_001159871.1
Coordinates 121860..122165 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag L3Z43_RS24005 Protein ID WP_000813630.1
Coordinates 122167..122385 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS23985 (117822) 117822..118988 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
L3Z43_RS23990 (119576) 119576..120331 - 756 WP_000852146.1 replication initiation protein RepE -
L3Z43_RS23995 (121053) 121053..121859 - 807 WP_000016970.1 site-specific integrase -
L3Z43_RS24000 (121860) 121860..122165 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
L3Z43_RS24005 (122167) 122167..122385 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L3Z43_RS24010 (122945) 122945..123175 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
L3Z43_RS24015 (123172) 123172..123588 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
L3Z43_RS24020 (123663) 123663..125228 + 1566 WP_001128474.1 AAA family ATPase -
L3Z43_RS24025 (125213) 125213..126235 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..145445 145445
- flank IS/Tn - - 126489..126992 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T233334 WP_001159871.1 NZ_CP091428:c122165-121860 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T233334 NZ_HG813240:362463-362888 [Mycobacterium tuberculosis 49-02]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT233334 WP_000813630.1 NZ_CP091428:c122385-122167 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT233334 NZ_HG813240:362245-362466 [Mycobacterium tuberculosis 49-02]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References