Detailed information of TA system TA233323

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3812189..3813023
• Replicon: chromosome
• Accession: NZ_CP091427
• Organism: Escherichia coli strain 1000C-3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: L3Z43_RS18245
• Protein ID: WP_000854820.1
• Coordinates: 3812646..3813023 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: L3Z43_RS18240
• Protein ID: WP_001285610.1
• Coordinates: 3812189..3812557 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Z43_RS18205 (3807910)	3807910..3808590	+	681	WP_001278649.1	WYL domain-containing protein	-
L3Z43_RS18210 (3808741)	3808741..3809418	+	678	WP_001097565.1	hypothetical protein	-
L3Z43_RS18215 (3809424)	3809424..3809657	+	234	WP_000883181.1	DUF905 family protein	-
L3Z43_RS18220 (3809747)	3809747..3810565	+	819	WP_001175155.1	DUF932 domain-containing protein	-
L3Z43_RS18225 (3810831)	3810831..3811310	+	480	WP_000706978.1	antirestriction protein	-
L3Z43_RS18230 (3811325)	3811325..3811801	+	477	WP_001186193.1	RadC family protein	-
L3Z43_RS18235 (3811888)	3811888..3812109	+	222	WP_000692347.1	DUF987 domain-containing protein	-
L3Z43_RS18240 (3812189)	3812189..3812557	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L3Z43_RS18245 (3812646)	3812646..3813023	+	378	WP_000854820.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
L3Z43_RS18250 (3813020)	3813020..3813217	+	198	Protein_3576	DUF5983 family protein	-
L3Z43_RS18255 (3813245)	3813245..3813442	+	198	WP_085949158.1	DUF957 domain-containing protein	-
L3Z43_RS18260 (3813527)	3813527..3814087	+	561	Protein_3578	DUF4942 domain-containing protein	-
L3Z43_RS18265 (3814454)	3814454..3814990	-	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
L3Z43_RS18270 (3814992)	3814992..3816170	-	1179	WP_000094991.1	type II secretion system protein GspL	-
L3Z43_RS18275 (3816167)	3816167..3817144	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
L3Z43_RS18280 (3817147)	3817147..3817746	-	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T233323 WP_000854820.1 NZ_CP091427:3812646-3813023 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T233323 NZ_HG738868:c1139726-1139523 [Serratia marcescens SMB2099]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCGGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT233323 WP_001285610.1 NZ_CP091427:3812189-3812557 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT233323 NZ_HG738868:c1140144-1139776 [Serratia marcescens SMB2099]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCACCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCAGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6