Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1102802..1103420 Replicon chromosome
Accession NZ_CP091427
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L3Z43_RS05250 Protein ID WP_001291435.1
Coordinates 1102802..1103020 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L3Z43_RS05255 Protein ID WP_000344800.1
Coordinates 1103046..1103420 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS05215 (1098091) 1098091..1098663 + 573 WP_000779831.1 YbaY family lipoprotein -
L3Z43_RS05220 (1098694) 1098694..1099005 - 312 WP_000409911.1 MGMT family protein -
L3Z43_RS05230 (1099384) 1099384..1099737 + 354 WP_000878140.1 DUF1428 domain-containing protein -
L3Z43_RS05235 (1099779) 1099779..1101329 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L3Z43_RS05240 (1101493) 1101493..1101963 - 471 WP_000136192.1 YlaC family protein -
L3Z43_RS05245 (1102079) 1102079..1102630 - 552 WP_000102564.1 maltose O-acetyltransferase -
L3Z43_RS05250 (1102802) 1102802..1103020 - 219 WP_001291435.1 HHA domain-containing protein Toxin
L3Z43_RS05255 (1103046) 1103046..1103420 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L3Z43_RS05260 (1103966) 1103966..1107115 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L3Z43_RS05265 (1107138) 1107138..1108331 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T233309 WP_001291435.1 NZ_CP091427:c1103020-1102802 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T233309 NZ_HG738867:c3505283-3504819 [Escherichia coli str. K-12 substr. MC4100]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT233309 WP_000344800.1 NZ_CP091427:c1103420-1103046 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT233309 NZ_HG738867:c3505618-3505283 [Escherichia coli str. K-12 substr. MC4100]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References