Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 387796..388391 Replicon chromosome
Accession NZ_CP091427
Organism Escherichia coli strain 1000C-3

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag L3Z43_RS01840 Protein ID WP_000239579.1
Coordinates 388041..388391 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag L3Z43_RS01835 Protein ID WP_001223208.1
Coordinates 387796..388047 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Z43_RS01825 (383461) 383461..387240 + 3780 WP_000060978.1 autotransporter assembly complex protein TamB -
L3Z43_RS01830 (387243) 387243..387584 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
L3Z43_RS01835 (387796) 387796..388047 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
L3Z43_RS01840 (388041) 388041..388391 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
L3Z43_RS01845 (388471) 388471..389001 - 531 WP_000055072.1 inorganic diphosphatase -
L3Z43_RS01850 (389311) 389311..390267 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
L3Z43_RS01855 (390407) 390407..391909 + 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
L3Z43_RS01860 (391923) 391923..392945 + 1023 WP_001295197.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T233304 WP_000239579.1 NZ_CP091427:388041-388391 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T233304 NZ_HG738867:c1811736-1811633 [Escherichia coli str. K-12 substr. MC4100]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT233304 WP_001223208.1 NZ_CP091427:387796-388047 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT233304 NZ_HG738867:1811595-1811866 [Escherichia coli str. K-12 substr. MC4100]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References