Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 210337..210923 Replicon plasmid unnamed1
Accession NZ_CP091425
Organism Methylobacter sp. YRD-M1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LZ558_RS21740 Protein ID WP_194970195.1
Coordinates 210337..210675 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag LZ558_RS21745 Protein ID WP_268120905.1
Coordinates 210675..210923 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ558_RS21715 (LZ558_21655) 205872..207551 - 1680 WP_268120900.1 tyrosine-type recombinase/integrase -
LZ558_RS21720 (LZ558_21660) 207548..208465 - 918 WP_268120901.1 DNA-binding protein -
LZ558_RS21725 (LZ558_21665) 208642..209052 + 411 WP_268120902.1 hypothetical protein -
LZ558_RS21730 (LZ558_21670) 209082..209744 + 663 WP_268120903.1 3'-5' exonuclease -
LZ558_RS21735 (LZ558_21675) 209799..209969 + 171 WP_268120904.1 hypothetical protein -
LZ558_RS21740 (LZ558_21680) 210337..210675 - 339 WP_194970195.1 endoribonuclease MazF Toxin
LZ558_RS21745 (LZ558_21685) 210675..210923 - 249 WP_268120905.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LZ558_RS21750 (LZ558_21690) 210990..211184 - 195 WP_194970197.1 hypothetical protein -
LZ558_RS21755 (LZ558_21695) 211546..211701 - 156 WP_268120906.1 hypothetical protein -
LZ558_RS21760 (LZ558_21700) 211727..212086 - 360 WP_194970199.1 hypothetical protein -
LZ558_RS21765 (LZ558_21705) 212034..213017 - 984 WP_268120907.1 PRTRC system protein D -
LZ558_RS21770 (LZ558_21710) 213640..213846 - 207 WP_194970201.1 hypothetical protein -
LZ558_RS21775 (LZ558_21715) 214435..215130 + 696 WP_268120908.1 antA/AntB antirepressor family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..349359 349359


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

SpoVT-AbrB domain

(6-48)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 11858.69 Da        Isoelectric Point: 7.1640

>T233300 WP_194970195.1 NZ_CP091425:c210675-210337 [Methylobacter sp. YRD-M1]
MAATYIPERGDLVWIAFDPQAGHEQAGRRPGLVITPHAYNAKAGLALICPVTSQVKGYPFEVALPVGGRVGGVVLADQVR
SLDWAARQAEKASKVDGNVLAEVLAKIEALIQ

Download         Length: 339 bp

>T233300 NZ_HG738867:c1302456-1302262 [Escherichia coli str. K-12 substr. MC4100]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8898.17 Da        Isoelectric Point: 5.6558

>AT233300 WP_268120905.1 NZ_CP091425:c210923-210675 [Methylobacter sp. YRD-M1]
MHTEIKKWGNSAVVRLPASMLARLKLEVGSPVELTVDDAGFRIVPTAAKPVFKLDDLLAGITADNRHDSIEWGPDVGRES
TS

Download         Length: 249 bp

>AT233300 NZ_HG738867:1302085-1302263 [Escherichia coli str. K-12 substr. MC4100]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References