Detailed information of TA system TA023330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1683369..1683895
• Replicon: chromosome
• Accession: NC_011741
• Organism: Escherichia coli IAI1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECIAI1_RS08170
• Protein ID: WP_000323026.1
• Coordinates: 1683369..1683656 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: ECIAI1_RS08175
• Protein ID: WP_000534858.1
• Coordinates: 1683656..1683895 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI1_RS08130	1678393..1678608	-	216	WP_000839590.1	phage lysis protein EssD	-
ECIAI1_RS08135	1679362..1679577	-	216	WP_000066484.1	cold shock-like protein CspB	-
ECIAI1_RS08140	1679878..1680090	+	213	WP_000087756.1	cold shock-like protein CspF	-
ECIAI1_RS25140	1680145..1680234	+	90	WP_120795389.1	hypothetical protein	-
ECIAI1_RS08145	1680512..1681264	-	753	WP_001047135.1	antitermination protein	-
ECIAI1_RS08150	1681278..1682327	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
ECIAI1_RS08155	1682329..1682607	-	279	WP_012304870.1	hypothetical protein	-
ECIAI1_RS08160	1682674..1682925	-	252	WP_000980994.1	hypothetical protein	-
ECIAI1_RS08165	1683142..1683297	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
ECIAI1_RS08170	1683369..1683656	-	288	WP_000323026.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
ECIAI1_RS08175	1683656..1683895	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
ECIAI1_RS23615	1683920..1684225	+	306	WP_001326990.1	hypothetical protein	-
ECIAI1_RS08180	1684428..1684760	+	333	WP_001317460.1	FlxA-like family protein	-
ECIAI1_RS08185	1685197..1686538	-	1342	Protein_1560	ISNCY family transposase	-
ECIAI1_RS25525	1687307..1687594	-	288	Protein_1561	hypothetical protein	-
ECIAI1_RS08200	1688205..1688561	-	357	WP_001374839.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1647983..1702192	54209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11209.18 Da
• Isoelectric Point: 10.1967

>T23330 WP_000323026.1 NC_011741:c1683656-1683369 [Escherichia coli IAI1]
MAYFLDFDERALKEWRKPGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T23330 NC_011741:c1683656-1683369 [Escherichia coli IAI1]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCCGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT23330 WP_000534858.1 NC_011741:c1683895-1683656 [Escherichia coli IAI1]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT23330 NC_011741:c1683895-1683656 [Escherichia coli IAI1]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A4V1CTS8