Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1683369..1683895 Replicon chromosome
Accession NC_011741
Organism Escherichia coli IAI1

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag ECIAI1_RS08170 Protein ID WP_000323026.1
Coordinates 1683369..1683656 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECIAI1_RS08175 Protein ID WP_000534858.1
Coordinates 1683656..1683895 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI1_RS08130 1678393..1678608 - 216 WP_000839590.1 phage lysis protein EssD -
ECIAI1_RS08135 1679362..1679577 - 216 WP_000066484.1 cold shock-like protein CspB -
ECIAI1_RS08140 1679878..1680090 + 213 WP_000087756.1 cold shock-like protein CspF -
ECIAI1_RS25140 1680145..1680234 + 90 WP_120795389.1 hypothetical protein -
ECIAI1_RS08145 1680512..1681264 - 753 WP_001047135.1 antitermination protein -
ECIAI1_RS08150 1681278..1682327 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ECIAI1_RS08155 1682329..1682607 - 279 WP_012304870.1 hypothetical protein -
ECIAI1_RS08160 1682674..1682925 - 252 WP_000980994.1 hypothetical protein -
ECIAI1_RS08165 1683142..1683297 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECIAI1_RS08170 1683369..1683656 - 288 WP_000323026.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECIAI1_RS08175 1683656..1683895 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECIAI1_RS23615 1683920..1684225 + 306 WP_001326990.1 hypothetical protein -
ECIAI1_RS08180 1684428..1684760 + 333 WP_001317460.1 FlxA-like family protein -
ECIAI1_RS08185 1685197..1686538 - 1342 Protein_1560 ISNCY family transposase -
ECIAI1_RS25525 1687307..1687594 - 288 Protein_1561 hypothetical protein -
ECIAI1_RS08200 1688205..1688561 - 357 WP_001374839.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1647983..1702192 54209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.18 Da        Isoelectric Point: 10.1967

>T23330 WP_000323026.1 NC_011741:c1683656-1683369 [Escherichia coli IAI1]
MAYFLDFDERALKEWRKPGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T23330 NC_011741:c1683656-1683369 [Escherichia coli IAI1]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCCGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT23330 WP_000534858.1 NC_011741:c1683895-1683656 [Escherichia coli IAI1]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT23330 NC_011741:c1683895-1683656 [Escherichia coli IAI1]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References