Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1210257..1211174 Replicon chromosome
Accession NZ_CP091421
Organism Bacillus sp. R45

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag L3V45_RS06350 Protein ID WP_003154806.1
Coordinates 1210428..1211174 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag L3V45_RS06345 Protein ID WP_003154807.1
Coordinates 1210257..1210427 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3V45_RS06305 (L3V45_06305) 1205488..1207110 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
L3V45_RS06310 (L3V45_06310) 1207123..1207494 + 372 WP_236837303.1 XkdW family protein -
L3V45_RS06315 (L3V45_06315) 1207500..1207697 + 198 WP_003154819.1 XkdX family protein -
L3V45_RS06320 (L3V45_06320) 1207754..1208515 + 762 WP_024085194.1 hypothetical protein -
L3V45_RS06325 (L3V45_06325) 1208567..1208830 + 264 WP_032866112.1 hemolysin XhlA family protein -
L3V45_RS06330 (L3V45_06330) 1208844..1209107 + 264 WP_003154813.1 phage holin -
L3V45_RS06335 (L3V45_06335) 1209121..1209999 + 879 WP_024085195.1 N-acetylmuramoyl-L-alanine amidase -
L3V45_RS06340 (L3V45_06340) 1210035..1210160 - 126 WP_003154809.1 hypothetical protein -
L3V45_RS06345 (L3V45_06345) 1210257..1210427 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
L3V45_RS06350 (L3V45_06350) 1210428..1211174 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
L3V45_RS06355 (L3V45_06355) 1211279..1212277 - 999 WP_003154805.1 inorganic phosphate transporter -
L3V45_RS06360 (L3V45_06360) 1212290..1212907 - 618 WP_003154804.1 DUF47 domain-containing protein -
L3V45_RS06365 (L3V45_06365) 1213193..1214509 - 1317 WP_046559522.1 amino acid permease -
L3V45_RS06370 (L3V45_06370) 1214832..1215782 + 951 WP_236837304.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T233284 WP_003154806.1 NZ_CP091421:c1211174-1210428 [Bacillus sp. R45]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T233284 NZ_HG326223:850928-851236 [Serratia marcescens subsp. marcescens Db11]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCCCTCTATGCTTTGGATAACGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCTATCGATTTTCTGATTGATGGCTTTTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT233284 WP_003154807.1 NZ_CP091421:c1210427-1210257 [Bacillus sp. R45]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT233284 NZ_HG326223:850689-850925 [Serratia marcescens subsp. marcescens Db11]
ATGGCAAATAGCAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCCGTATTGACGGACGCATTGAGCGTCCGCGTCAGGGATATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGCGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References