Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 472647..473284 Replicon chromosome
Accession NZ_CP091421
Organism Bacillus sp. R45

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag L3V45_RS02420 Protein ID WP_003156187.1
Coordinates 472934..473284 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag L3V45_RS02415 Protein ID WP_003156188.1
Coordinates 472647..472928 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3V45_RS02395 (L3V45_02395) 469012..469611 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
L3V45_RS02400 (L3V45_02400) 469704..470069 + 366 WP_003156192.1 holo-ACP synthase -
L3V45_RS02405 (L3V45_02405) 470234..471241 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
L3V45_RS02410 (L3V45_02410) 471358..472527 + 1170 WP_003156189.1 alanine racemase -
L3V45_RS02415 (L3V45_02415) 472647..472928 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
L3V45_RS02420 (L3V45_02420) 472934..473284 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L3V45_RS02425 (L3V45_02425) 473402..474223 + 822 WP_003156182.1 STAS domain-containing protein -
L3V45_RS02430 (L3V45_02430) 474228..474593 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
L3V45_RS02435 (L3V45_02435) 474596..474997 + 402 WP_003156178.1 anti-sigma regulatory factor -
L3V45_RS02440 (L3V45_02440) 475009..476016 + 1008 WP_025853725.1 PP2C family protein-serine/threonine phosphatase -
L3V45_RS02445 (L3V45_02445) 476080..476409 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
L3V45_RS02450 (L3V45_02450) 476406..476888 + 483 WP_003156173.1 anti-sigma B factor RsbW -
L3V45_RS02455 (L3V45_02455) 476854..477642 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
L3V45_RS02460 (L3V45_02460) 477642..478244 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T233283 WP_003156187.1 NZ_CP091421:472934-473284 [Bacillus sp. R45]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T233283 NZ_HG326223:c389198-388995 [Serratia marcescens subsp. marcescens Db11]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT233283 WP_003156188.1 NZ_CP091421:472647-472928 [Bacillus sp. R45]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT233283 NZ_HG326223:c389617-389249 [Serratia marcescens subsp. marcescens Db11]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGCTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GATGATACCTATACGCTGTTCAGCAGTTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References