Detailed information of TA system TA233281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 626998..627774
• Replicon: chromosome
• Accession: NZ_CP091412
• Organism: Staphylococcus aureus strain ATR-20003

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: L3I92_RS03415
• Protein ID: WP_000031109.1
• Coordinates: 627622..627774 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: L3I92_RS03410
• Protein ID: WP_001251224.1
• Coordinates: 626998..627597 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3I92_RS03390 (622914)	622914..624371	+	1458	WP_000649917.1	ABC transporter permease subunit	-
L3I92_RS03395 (624364)	624364..625086	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
L3I92_RS03400 (625237)	625237..626364	+	1128	WP_049295390.1	tRNA epoxyqueuosine(34) reductase QueG	-
L3I92_RS03405 (626369)	626369..626839	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
L3I92_RS03410 (626998)	626998..627597	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
L3I92_RS03415 (627622)	627622..627774	+	153	WP_000031109.1	SAS053 family protein	Toxin
L3I92_RS03420 (628542)	628542..628937	+	396	WP_000901019.1	hypothetical protein	-
L3I92_RS03425 (629133)	629133..630518	+	1386	WP_000116239.1	class II fumarate hydratase	-
L3I92_RS03430 (630975)	630975..631796	-	822	WP_000669377.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5950.31 Da
• Isoelectric Point: 3.9075

>T233281 WP_000031109.1 NZ_CP091412:627622-627774 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEESQ

Nucleotide

• Length: 153 bp

>T233281 NZ_HG322950:5105771-5106025 [Pseudomonas knackmussii B13]
ATGAACAGCCGCCAACGCTCCACCTGGGAGCTGTTGTACGCGACGCCAACGCCCAACCACCTGGAATGGTTCCGGATCGA
GGCACTGCTGCTGTACCTGGGCGCGCGCAAGATCGAGGGCAACGGTTCGCGCGTGCGCTTCGAGCTGAACGGCGTCATCG
CCAGCTTCCACCGGCCACATCCGGGCAAGGAAGCCAAACCCTATCAGGTTCGCGATGCGCGGACTTTCCTCGAACGAGCG
GGATACGCACCATGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT233281 WP_001251224.1 NZ_CP091412:626998-627597 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT233281 NZ_HG322950:5106022-5106348 [Pseudomonas knackmussii B13]
ATGAGCCCGATGAAATACAAAGGCTACGTCGCCCACATCGAATACAGCGACGACGATGAACTGTTCGTTGGCCGCATCGC
CGGCATCCACGACGTGATCGGTTTCCATGGCGAGTCGGTAGGCGAGCTGCGCCAACTGTTCCAGGAAGCGGTCGACGACT
ACCTGGAGACCTGCGCTCGACTCGGTCGTGAACCGCAGAAGCCCTACTCAGGAAAACTCAGCCTGCGCCTGGATCCCGCC
CTCCACGCACGCGTAGCCCTGAAGGCCGAGCTGAGCAATAAGAGCATCAACCAGTGGGTGGTGGAGCGCCTCAGCGAGGC
GCTCTGA

Structures

Antitoxin

• AlphaFold DB: W8U4V4