23328

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 494847..495465 Replicon chromosome
Accession NC_011741
Organism Escherichia coli IAI1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECIAI1_RS02305 Protein ID WP_001291435.1
Coordinates 494847..495065 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECIAI1_RS02310 Protein ID WP_000344800.1
Coordinates 495091..495465 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI1_RS02275 490137..490709 + 573 WP_000779831.1 YbaY family lipoprotein -
ECIAI1_RS02280 490740..491051 - 312 WP_000409911.1 MGMT family protein -
ECIAI1_RS02285 491430..491783 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECIAI1_RS02290 491825..493375 - 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECIAI1_RS02295 493539..494009 - 471 WP_000136192.1 YlaC family protein -
ECIAI1_RS02300 494125..494676 - 552 WP_000102578.1 maltose O-acetyltransferase -
ECIAI1_RS02305 494847..495065 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECIAI1_RS02310 495091..495465 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECIAI1_RS02315 496011..499160 - 3150 WP_001132490.1 efflux RND transporter permease AcrB -
ECIAI1_RS02320 499183..500376 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T23328 WP_001291435.1 NC_011741:c495065-494847 [Escherichia coli IAI1]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T23328 NC_011741:c495065-494847 [Escherichia coli IAI1]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT23328 WP_000344800.1 NC_011741:c495465-495091 [Escherichia coli IAI1]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT23328 NC_011741:c495465-495091 [Escherichia coli IAI1]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References