Detailed information of TA system TA233276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 431433..431962
• Replicon: chromosome
• Accession: NZ_CP091412
• Organism: Staphylococcus aureus strain ATR-20003

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L3I92_RS02195
• Protein ID: WP_000621175.1
• Coordinates: 431600..431962 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: L3I92_RS02190
• Protein ID: WP_000948331.1
• Coordinates: 431433..431603 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3I92_RS02160 (426470)	426470..427030	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
L3I92_RS02165 (427238)	427238..427717	+	480	WP_001287077.1	hypothetical protein	-
L3I92_RS02170 (427710)	427710..429293	+	1584	WP_001294637.1	PH domain-containing protein	-
L3I92_RS02175 (429280)	429280..429771	+	492	WP_001205907.1	PH domain-containing protein	-
L3I92_RS02180 (429775)	429775..430134	+	360	WP_000581199.1	holo-ACP synthase	-
L3I92_RS02185 (430200)	430200..431348	+	1149	WP_001281140.1	alanine racemase	-
L3I92_RS02190 (431433)	431433..431603	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L3I92_RS02195 (431600)	431600..431962	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L3I92_RS02200 (432311)	432311..433312	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
L3I92_RS02205 (433431)	433431..433757	+	327	WP_001052491.1	anti-sigma factor antagonist	-
L3I92_RS02210 (433759)	433759..434238	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
L3I92_RS02215 (434213)	434213..434983	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T233276 WP_000621175.1 NZ_CP091412:431600-431962 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T233276 NZ_HG322950:c54346-54065 [Pseudomonas knackmussii B13]
ATGAGCCTGCAGTGGACGCACAAGGCGGCCGCCGACCTGGACGGCCTGTACGACCACTACGTGGTGCTGGTCGGCCCGGA
GAAGGCCCTCCGGGCGATCCAGGACATGGTCAGCCAGGTGAAGGCGCTGGCCGATCTCAGCCTGAGCAGTGTCGGCCAGC
CGAGCGAAGTGCCCGGGGTGCGCGAATTGCTGCTCGAACGTTGGCCTTACCAAGCCGCGTACCGGGTAAAGGGCCGCGAT
GTACAAATTTTGCGCATCGATGCTGTGGAAAACTCGGGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT233276 WP_000948331.1 NZ_CP091412:431433-431603 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT233276 NZ_HG322950:c54570-54343 [Pseudomonas knackmussii B13]
TTGACCACCGTAATCTCCTTCCGTGCCGATGACACCCTGGCCGCAGCCCTGGATCAGCTGGCCCGCGCCACCCATCGGGA
CCGCCCGTACCACCTGCGCCAGGCCCTGGCGCAGTACCTGGAGCGCCAGGCCTGGCACGTCGCAGCCATCGACGAGGGGC
TGGCCGACGCCAATGCCGGGCATCTGCTGGACCACGTGGAAATCGAACGCAAATGGGGGCTGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1