233226

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 3015552..3016140 Replicon chromosome
Accession NZ_CP091356
Organism Acinetobacter baumannii strain AB186-VUB

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag L2Z19_RS14350 Protein ID WP_000438825.1
Coordinates 3015552..3015839 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag L2Z19_RS14355 Protein ID WP_004728120.1
Coordinates 3015826..3016140 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Z19_RS14315 (L2Z19_14300) 3011310..3011582 - 273 WP_000369781.1 NadS family protein -
L2Z19_RS14320 (L2Z19_14305) 3011575..3011895 - 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
L2Z19_RS14325 (L2Z19_14310) 3012024..3013358 - 1335 WP_033917544.1 ISNCY family transposase -
L2Z19_RS14330 (L2Z19_14315) 3013521..3013940 + 420 WP_001180106.1 SMI1/KNR4 family protein -
L2Z19_RS14335 (L2Z19_14320) 3014020..3014301 - 282 WP_000985609.1 putative addiction module antidote protein -
L2Z19_RS14340 (L2Z19_14325) 3014302..3014604 - 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
L2Z19_RS14345 (L2Z19_14330) 3014753..3015283 + 531 WP_000172759.1 hypothetical protein -
L2Z19_RS14350 (L2Z19_14335) 3015552..3015839 + 288 WP_000438825.1 BrnT family toxin Toxin
L2Z19_RS14355 (L2Z19_14340) 3015826..3016140 + 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
L2Z19_RS14360 (L2Z19_14345) 3016201..3016971 - 771 WP_032073134.1 hypothetical protein -
L2Z19_RS14365 (L2Z19_14350) 3017030..3017368 + 339 WP_032073153.1 hypothetical protein -
L2Z19_RS14370 (L2Z19_14355) 3017496..3018200 - 705 WP_071543226.1 IS6-like element IS1006 family transposase -
L2Z19_RS14375 (L2Z19_14360) 3018264..3019094 + 831 Protein_2781 IS91-like element ISVsa3 family transposase -
L2Z19_RS14380 (L2Z19_14365) 3019270..3019572 - 303 WP_000251875.1 phosphoglucosamine mutase -
L2Z19_RS14385 (L2Z19_14370) 3019659..3020474 - 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
L2Z19_RS14390 (L2Z19_14375) 3020564..3020911 + 348 Protein_2784 IS4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island sul2 - 3012024..3020962 8938
- inside IScluster/Tn blaADC-25 / mph(E) / msr(E) / sul2 - 3002887..3021694 18807


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T233226 WP_000438825.1 NZ_CP091356:3015552-3015839 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T233226 NZ_FO834906:786979-787389 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT233226 WP_004728120.1 NZ_CP091356:3015826-3016140 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT233226 NZ_FO834906:786732-786998 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References