Detailed information of TA system TA233212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5819..6407
• Replicon: chromosome
• Accession: NZ_CP091350
• Organism: Acinetobacter baumannii strain AB212-VUB

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: L2Z25_RS00040
• Protein ID: WP_000438826.1
• Coordinates: 5819..6106 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: L2Z25_RS00045
• Protein ID: WP_001983304.1
• Coordinates: 6093..6407 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Z25_RS00015 (L2Z25_00015)	1269..2097	-	829	Protein_2	AraC family transcriptional regulator	-
L2Z25_RS00020 (L2Z25_00020)	2271..2977	-	707	Protein_3	IS1 family transposase	-
L2Z25_RS00025 (L2Z25_00025)	3068..3910	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
L2Z25_RS00030 (L2Z25_00030)	4145..5107	+	963	WP_004746678.1	IS30-like element IS18 family transposase	-
L2Z25_RS00035 (L2Z25_00035)	5104..5589	+	486	WP_220385121.1	LysE family transporter	-
L2Z25_RS00040 (L2Z25_00040)	5819..6106	+	288	WP_000438826.1	BrnT family toxin	Toxin
L2Z25_RS00045 (L2Z25_00045)	6093..6407	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
L2Z25_RS00050 (L2Z25_00050)	6534..8944	+	2411	Protein_9	TonB-dependent receptor ZnuD2	-
L2Z25_RS00055 (L2Z25_00055)	9249..9710	+	462	WP_000761346.1	DIP1984 family protein	-
L2Z25_RS00060 (L2Z25_00060)	10033..10206	-	174	WP_001282484.1	hypothetical protein	-
L2Z25_RS00065 (L2Z25_00065)	10206..10577	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T233212 WP_000438826.1 NZ_CP091350:5819-6106 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T233212 NZ_FO818637:3828645-3829022 [Xenorhabdus bovienii]
ATGCGAGTGCGAGTTATCACTGCACGAGTCATCATTGAAGTAATGAGGAAACATGCTCAATGGAAAGTTGGGTTAAAGCT
ATGGCTGGATGTTTTTGATAAAGCAGCTTTGCGGTTTGAATCTTATGCCCAGATACGTGATCTCTGGCTTCAAACATCTG
CATGGAACGTTGATCGTATACCTCATGAGCTATTGGAGGCAGGGAATCAGAAGGGGCCGCTTGATATTTATATATTTGAT
ATTCATAAAAATAAATGCAGGATAATTGCATGGCTTAACCCCAAATCCGGTACTTTTTATATTAAAGATGTTTGCTCCCA
TGCTAAATACGATAGATGGTGGCGTGAACAAACCAAATCAAACAGGCCAAAAAGGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT233212 WP_001983304.1 NZ_CP091350:6093-6407 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT233212 NZ_FO818637:3829026-3829496 [Xenorhabdus bovienii]
ATGAGACAAGCATTACTCGTTTATGAAGAAATTGATGGGATCATCAAGAAATTACCGCAAATGATGCAGTCAGTATCTGA
TCAACTCAGCCCTCTTGCCCTTTTATTTTCCGCCTGCCTTGAACCCGTAGAGAACGAAGAAGATCTTAAAGCTCGGATGG
TCATAATTGATGAATTGTATTCATATGCCGATGATACTGAGCATGTAGCAGCCAAATTCGCCGATTTTGTCACAGATCGC
ATTTATGAATATGAAACCAAAAACCGGAATGTATCTAACGTCAGCCCGCGTGAAGCACTGGCTTTTTTTATGAAAGAACG
GAGGATCAGGCAAGCTGATCTGTGTGATATTGCCACTCAAAGTGTGATATCAGAGATCCTTCACGGAAAGCGTTCAATGA
CAATTCAACAAGTGAAAGGGTTTGCTAAATTTTTTGGGGTTCCCGTTGAAACGTTTATGGGAACGCTATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3