Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 57642..58285 Replicon plasmid pCRK3022-2
Accession NZ_CP091332
Organism Klebsiella pneumoniae strain CRK3022

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag L3Y35_RS27455 Protein ID WP_001044770.1
Coordinates 57869..58285 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag L3Y35_RS27450 Protein ID WP_001261282.1
Coordinates 57642..57872 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y35_RS27410 52847..53149 + 303 WP_004197636.1 hypothetical protein -
L3Y35_RS27415 53605..54399 - 795 WP_004197635.1 site-specific integrase -
L3Y35_RS27420 54597..55613 - 1017 WP_017899884.1 hypothetical protein -
L3Y35_RS27425 55624..55938 - 315 WP_053389906.1 hypothetical protein -
L3Y35_RS27430 55965..56360 - 396 WP_017899885.1 hypothetical protein -
L3Y35_RS27435 56529..56834 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
L3Y35_RS27440 56836..57054 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
L3Y35_RS27445 57224..57685 - 462 WP_160880186.1 hypothetical protein -
L3Y35_RS27450 57642..57872 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L3Y35_RS27455 57869..58285 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
L3Y35_RS27460 58359..59921 + 1563 WP_004206609.1 AAA family ATPase -
L3Y35_RS27465 59906..60928 + 1023 WP_000361404.1 helicase UvrD -
L3Y35_RS27470 61184..61881 + 698 WP_223174630.1 IS1 family transposase -
L3Y35_RS27475 61910..62182 + 273 Protein_72 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..123020 123020


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T233187 WP_001044770.1 NZ_CP091332:57869-58285 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T233187 NZ_FO704550:c128701-128303 [Xenorhabdus doucetiae]
ATGATATGGGTCAGCGCACAGGAAGTTATCGCTTTTCATGACCGAATATTACAACGGCTTCCCGGCGTTGCCGGAATGCC
CGATTCTGGTAGAGCTGAAGCACTCATCTATCGGGTACAAAACCGTACACACTATGAAGGCATAACAGATATTTTTGAAC
TTGCAGCGACCTATTGGGCTGCTATCGCTCGTGGTCATATTTTCAATGATGGTAATAAGCGCACAGCGTTTTTCGTTACC
ATGACTTTTCTCTATCGTAACGGTATCAAGATCCTTGATAACGACAATACATTGGAAAACTTAACAGTAGATGCCGCTAC
GGGCGAGAAAACCGTTGACCAGTTGGCGCAGCACCTACGCGAATTGGTGGACTATACTAATCTGAGCTGTAATCCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT233187 WP_001261282.1 NZ_CP091332:57642-57872 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT233187 NZ_FO704550:c128919-128698 [Xenorhabdus doucetiae]
ATGCGTACATACACCTCAACTCAAGCCCGTGCCAACATCGCGGAAGTGTTGGACACAGCGGCTCACGGTGAACCTGTTGA
AATTACCCGTCGAGACGGCAGCTCAGCAGTCGTTATCAGTAAAGCAGAATTCGATGCTTGGCAAAACGCTAAGCTGGATG
CAGAGTTCGATGCGATTATGCAACGTCATGGGCATACGATAGAGGCGCTGACAAATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References