Detailed information of TA system TA233186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 56529..57054
• Replicon: plasmid pCRK3022-2
• Accession: NZ_CP091332
• Organism: Klebsiella pneumoniae strain CRK3022

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: L3Y35_RS27435
• Protein ID: WP_013023785.1
• Coordinates: 56529..56834 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: L3Y35_RS27440
• Protein ID: WP_001568025.1
• Coordinates: 56836..57054 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Y35_RS27405	52224..52850	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
L3Y35_RS27410	52847..53149	+	303	WP_004197636.1	hypothetical protein	-
L3Y35_RS27415	53605..54399	-	795	WP_004197635.1	site-specific integrase	-
L3Y35_RS27420	54597..55613	-	1017	WP_017899884.1	hypothetical protein	-
L3Y35_RS27425	55624..55938	-	315	WP_053389906.1	hypothetical protein	-
L3Y35_RS27430	55965..56360	-	396	WP_017899885.1	hypothetical protein	-
L3Y35_RS27435	56529..56834	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
L3Y35_RS27440	56836..57054	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
L3Y35_RS27445	57224..57685	-	462	WP_160880186.1	hypothetical protein	-
L3Y35_RS27450	57642..57872	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	-
L3Y35_RS27455	57869..58285	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
L3Y35_RS27460	58359..59921	+	1563	WP_004206609.1	AAA family ATPase	-
L3Y35_RS27465	59906..60928	+	1023	WP_000361404.1	helicase UvrD	-
L3Y35_RS27470	61184..61881	+	698	WP_223174630.1	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..123020	123020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T233186 WP_013023785.1 NZ_CP091332:c56834-56529 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T233186 NZ_FO704550:c21449-20904 [Xenorhabdus doucetiae]
ATGGAAAAGTCACTAAATGAAATTAGGGTTGAATACATTTGTGTGAACTATCAGAGCGATATAACCTATCTGGGTCAAAA
AGCTTTTGATTGTGGCAATCATGTTATTAACCGTTATGTCCGCAGTAGTCTTAAAAGCAATGTCGCCAACGGAAATTGTG
TTGCTAAGGCTTTAATTGACGCTGAAACGGGTGAGTTGCTTGGTGTGTGCAGTTTTACCGGTTATTCACTGGCAAAATCT
CGTCTAAGTGGTGTACTAAGCGGTTCCATGCCAAGTGACCTTTCTGTTATTCGTTTGGTGATGTTGGGCGTATCGGTTAA
ACATCAAAAGAAAGGATATGGTCATATTTTAATGAGGGAATTTTTAGAGCACGCTATTAAAGTACATCAGGTCATGCCAA
TTAAAGGAATATTTTTGGATGCCGATCCTGACGCTGTTGCGTTTTACATACTTCTTGGATTTGTTGAGCTGGGAACGCCT
GCATCCAATGGAACAACTCCGATGTTTTTAAAAATTCAGGATTTATTGGCGGCTATACCTTATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT233186 WP_001568025.1 NZ_CP091332:c57054-56836 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT233186 NZ_FO704550:c21735-21430 [Xenorhabdus doucetiae]
ATGGCCACAATATCGGTAAAAAAACCTCAAAAAGAGGCTAAAAATGCTCGTGTAGAGTTAAAAACCAGTACCGAGCTAAA
AGAAACCTTGCGTCAGGCAGCACAGGTTGCTGGTGTTGATTTAAGTGCTTTTATCTTAAGTGCGGCTTTTGAACGAGCGG
AAGAAGTGCTGGAAAATCAAAAGCGTCGTGAATTAGATGAAGAAAACTGGCGTACCTTGAATGTACTGATTGCTGAACCT
GCCTCTCCAACTCTTGCCTTACGTGCTTTGATGAGAAAAAACAAAAATGGAAAAGTCACTAAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8