Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 113865..114601 Replicon plasmid pCRK3022-1
Accession NZ_CP091331
Organism Klebsiella pneumoniae strain CRK3022

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag L3Y35_RS26640 Protein ID WP_003026803.1
Coordinates 114119..114601 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L3Y35_RS26635 Protein ID WP_003026799.1
Coordinates 113865..114131 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y35_RS26590 109927..110289 - 363 WP_215402459.1 arsenite efflux transporter metallochaperone ArsD -
L3Y35_RS26595 110339..110689 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
L3Y35_RS26600 111047..111316 + 270 WP_004152102.1 hypothetical protein -
L3Y35_RS26605 111304..111879 + 576 WP_004152103.1 hypothetical protein -
L3Y35_RS26610 111910..112404 + 495 WP_004152104.1 DNA-binding protein -
L3Y35_RS26615 112448..112816 + 369 WP_004152105.1 hypothetical protein -
L3Y35_RS26620 112850..113053 + 204 WP_004152106.1 HHA domain-containing protein -
L3Y35_RS26625 113102..113359 + 258 WP_004152107.1 hypothetical protein -
L3Y35_RS26630 113435..113689 + 255 WP_004152108.1 hypothetical protein -
L3Y35_RS26635 113865..114131 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L3Y35_RS26640 114119..114601 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
L3Y35_RS26645 114813..116159 + 1347 WP_138920866.1 ISNCY family transposase -
L3Y35_RS27845 116320..116451 + 132 WP_004218042.1 hypothetical protein -
L3Y35_RS26650 116660..117825 - 1166 Protein_134 IS3 family transposase -
L3Y35_RS26655 118002..118964 - 963 WP_004152113.1 zinc metalloprotease HtpX -
L3Y35_RS26660 118951..119439 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / mph(A) - 1..203312 203312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T233185 WP_003026803.1 NZ_CP091331:114119-114601 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T233185 NZ_FO704549:c5397-5098 [Xenorhabdus doucetiae]
ATGAAACCAGAAATTGAGTTTGAATGGGATACCGTCAAAGCAGCAAGCAATTTCCGTAAGCATGGGGTTCGTTTTGAAGC
GGCTTCACGGGTTTTTGACGATCCCTTTCACCTGACAGTCCAGGATAGGTTTGAAAACGGCGAATACCGTTGGCAAACCA
TTGGCACAGTAGAAAACTGTGTGCTTGTTCTGGTGGCGCATACAACACGCTTTGAAGATGGCACTGAAATCATTCGCATC
ATCAGTGCCCGAAAAGCCGAGCGGCATGAGAGAAAACGTTATGAGCATGGTGAGATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT233185 WP_003026799.1 NZ_CP091331:113865-114131 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT233185 NZ_FO704549:c5117-4815 [Xenorhabdus doucetiae]
ATGAGCATGGTGAGATATAAACGAAACGAACTGCCTCCGCTAAGCGAAAAACAGAAAGCTGAACTGAGGAAACTTTCCGA
TAAGCCGGACAGTGATATTGATTACAGCGACATTGCGCCGCTGGATACTGCTTTCTGGAAGAACGCCACACAAGGACAGT
TTTATCGACCTGTAAAAACACAAACATCGGTCAGAATTGATGCGGATGTTCTTGCCTGGCTTAAGGAAACCGGAAAAGGT
TATCAGACGAGGCTTAATGCGATACTACGTGAAGCCATGCTGCGTGATCTGCATCGAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References