Detailed information of TA system TA233184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 39339..39982
• Replicon: plasmid pCRK3022-1
• Accession: NZ_CP091331
• Organism: Klebsiella pneumoniae strain CRK3022

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: L3Y35_RS26245
• Protein ID: WP_014386165.1
• Coordinates: 39566..39982 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: L3Y35_RS26240
• Protein ID: WP_032408893.1
• Coordinates: 39339..39569 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Y35_RS26215	34412..35395	-	984	WP_001471744.1	NAD(P)H-quinone oxidoreductase	-
L3Y35_RS26220	35414..36562	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
L3Y35_RS26225	36733..37890	-	1158	WP_032408895.1	acetylornithine deacetylase	-
L3Y35_RS26230	37906..38580	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
L3Y35_RS26235	38585..39019	-	435	WP_000103648.1	RidA family protein	-
L3Y35_RS26240	39339..39569	+	231	WP_032408893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L3Y35_RS26245	39566..39982	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
L3Y35_RS26250	40305..41273	+	969	WP_074422984.1	IS5-like element IS903B family transposase	-
L3Y35_RS26255	41453..41827	-	375	WP_032408891.1	hypothetical protein	-
L3Y35_RS26260	41883..42209	-	327	WP_004152639.1	hypothetical protein	-
L3Y35_RS26265	42206..42934	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
L3Y35_RS26270	42931..43361	-	431	Protein_57	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / mph(A)	-	1..203312	203312
	-	flank	IS/Tn	-	-	40350..41273	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T233184 WP_014386165.1 NZ_CP091331:39566-39982 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T233184 NZ_FO082822:12345-12617 [Pseudorhizobium banfieldiae]
GTGCGGGAACCGAGCTATTCCGGCCAGTTCCGCCGTGACGTGAAGCGGGCGGAAAAGCGCGGCAAGGACATGGCGAAGCT
GCGCCACGTCCTGCAATTGCTGATCGAGGGCCAGCCCTTGCCGGCGCAACTGCGTGATCACCCCTTGAAATCGAACTGGA
TCGGTTATCGCGACCTGCACATAGAGCCGGATTGGCTGCTGATCTACAAAGCGGACGCGACGAGCGTTCATTTCGAGCGC
ACCGGAACCCACTCCGATCTTTTCCGTAAGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT233184 WP_032408893.1 NZ_CP091331:39339-39569 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT233184 NZ_FO082822:12084-12344 [Pseudorhizobium banfieldiae]
ATGAGAGTTGATACCGTAGTGCGCGCGCGCGTCGATGCCGAAACGAAGGATAGCGCCACGAAGGCGCTCGCGGAAATGGG
ATTGTCCGTCTCTGATTATATCCGTATGGCGCTGGTCCGGGTTGCCCGCGATAAGGCCATCCCCTTTCCGGTGAAGGCTC
CGAATGCGAAGACGGCTGCGGCTATGGAGAAGACGGCGCGCGGCGAGGATGTTCACAAGGCGAAGGATGCCGACGACCTG
TTCAAGCAGTTGGACATTTGA