Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4045986..4046611 Replicon chromosome
Accession NZ_CP091330
Organism Klebsiella pneumoniae strain CRK3022

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L3Y35_RS19355 Protein ID WP_002882817.1
Coordinates 4045986..4046369 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L3Y35_RS19360 Protein ID WP_004150355.1
Coordinates 4046369..4046611 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y35_RS19340 (4043352) 4043352..4044254 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L3Y35_RS19345 (4044251) 4044251..4044886 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L3Y35_RS19350 (4044883) 4044883..4045812 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L3Y35_RS19355 (4045986) 4045986..4046369 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L3Y35_RS19360 (4046369) 4046369..4046611 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L3Y35_RS19365 (4046816) 4046816..4047733 + 918 WP_004181612.1 alpha/beta hydrolase -
L3Y35_RS19370 (4047747) 4047747..4048688 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L3Y35_RS19375 (4048733) 4048733..4049170 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L3Y35_RS19380 (4049167) 4049167..4050027 - 861 WP_004146232.1 virulence factor BrkB family protein -
L3Y35_RS19385 (4050021) 4050021..4050620 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T233180 WP_002882817.1 NZ_CP091330:c4046369-4045986 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T233180 NZ_CP091330:c4046369-4045986 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT233180 WP_004150355.1 NZ_CP091330:c4046611-4046369 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT233180 NZ_CP091330:c4046611-4046369 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References