Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2832040..2832659 Replicon chromosome
Accession NZ_CP091330
Organism Klebsiella pneumoniae strain CRK3022

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L3Y35_RS13675 Protein ID WP_002892050.1
Coordinates 2832441..2832659 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L3Y35_RS13670 Protein ID WP_002892066.1
Coordinates 2832040..2832414 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y35_RS13660 (2827192) 2827192..2828385 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L3Y35_RS13665 (2828408) 2828408..2831554 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
L3Y35_RS13670 (2832040) 2832040..2832414 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L3Y35_RS13675 (2832441) 2832441..2832659 + 219 WP_002892050.1 HHA domain-containing protein Toxin
L3Y35_RS13680 (2832818) 2832818..2833384 + 567 WP_002892030.1 maltose O-acetyltransferase -
L3Y35_RS13685 (2833356) 2833356..2833496 - 141 WP_004147370.1 hypothetical protein -
L3Y35_RS13690 (2833517) 2833517..2833987 + 471 WP_002892026.1 YlaC family protein -
L3Y35_RS13695 (2833962) 2833962..2835413 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
L3Y35_RS13700 (2835514) 2835514..2836212 + 699 WP_002892021.1 GNAT family protein -
L3Y35_RS13705 (2836209) 2836209..2836349 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L3Y35_RS13710 (2836349) 2836349..2836612 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T233176 WP_002892050.1 NZ_CP091330:2832441-2832659 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T233176 NZ_CP128551:481617-481967 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT233176 WP_002892066.1 NZ_CP091330:2832040-2832414 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT233176 NZ_CP128551:481330-481611 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References