Detailed information of TA system TA233174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1460706..1462294
• Replicon: chromosome
• Accession: NZ_CP091330
• Organism: Klebsiella pneumoniae strain CRK3022

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: L3Y35_RS07005
• Protein ID: WP_004179815.1
• Coordinates: 1460706..1462028 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: L3Y35_RS07010
• Protein ID: WP_004176198.1
• Coordinates: 1462028..1462294 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Y35_RS06990 (1455762)	1455762..1458263	+	2502	WP_020323802.1	fimbria/pilus outer membrane usher protein	-
L3Y35_RS06995 (1458251)	1458251..1459255	+	1005	WP_004179822.1	fimbrial protein	-
L3Y35_RS07000 (1459397)	1459397..1460689	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
L3Y35_RS07005 (1460706)	1460706..1462028	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
L3Y35_RS07010 (1462028)	1462028..1462294	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
L3Y35_RS07015 (1462560)	1462560..1462886	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
L3Y35_RS07020 (1462883)	1462883..1463383	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
L3Y35_RS07025 (1463361)	1463361..1464740	-	1380	WP_094932908.1	nucleobase:cation symporter-2 family protein	-
L3Y35_RS07030 (1465086)	1465086..1466240	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
L3Y35_RS07035 (1466221)	1466221..1467144	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T233174 WP_004179815.1 NZ_CP091330:c1462028-1460706 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T233174 NZ_CP128550:4997181-4997459 [Pseudomonas alloputida]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT233174 WP_004176198.1 NZ_CP091330:c1462294-1462028 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT233174 NZ_CP128550:4997468-4997767 [Pseudomonas alloputida]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA