Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 197856..198766 Replicon plasmid phvK2115
Accession NZ_CP091327
Organism Klebsiella pneumoniae strain hvK2115

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag L3Y29_RS26565 Protein ID WP_004026354.1
Coordinates 198296..198766 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag L3Y29_RS26560 Protein ID WP_004026357.1
Coordinates 197856..198299 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y29_RS26530 (L3Y29_26530) 192874..193395 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
L3Y29_RS26535 (L3Y29_26535) 193392..194345 + 954 Protein_216 ferric citrate uptake sigma factor regulator FecR -
L3Y29_RS26540 (L3Y29_26540) 194432..196558 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
L3Y29_RS26545 (L3Y29_26545) 196633..196758 + 126 Protein_218 Fe3+-citrate ABC transporter substrate-binding protein -
L3Y29_RS26550 (L3Y29_26550) 196933..197154 + 222 WP_004213643.1 DUF2188 domain-containing protein -
L3Y29_RS26555 (L3Y29_26555) 197252..197755 + 504 Protein_220 DUF4113 domain-containing protein -
L3Y29_RS26560 (L3Y29_26560) 197856..198299 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
L3Y29_RS26565 (L3Y29_26565) 198296..198766 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
L3Y29_RS26570 (L3Y29_26570) 199221..199409 - 189 WP_223271245.1 hypothetical protein -
L3Y29_RS26575 (L3Y29_26575) 200023..200283 - 261 WP_004026352.1 hypothetical protein -
L3Y29_RS26580 (L3Y29_26580) 200531..201577 - 1047 WP_159216731.1 IS481-like element ISKpn28 family transposase -
L3Y29_RS26585 (L3Y29_26585) 201649..201807 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
L3Y29_RS26590 (L3Y29_26590) 202186..202428 - 243 WP_071826037.1 hypothetical protein -
L3Y29_RS26595 (L3Y29_26595) 202504..202668 - 165 WP_011154586.1 hypothetical protein -
L3Y29_RS26600 (L3Y29_26600) 202785..202946 - 162 WP_164507110.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iucA / iucB / iucC / iucD / iutA / rmpA2 / rmpA / iroN / iroD / iroC / iroB 1..223967 223967
- flank IS/Tn - - 200531..201577 1046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T233160 WP_004026354.1 NZ_CP091327:198296-198766 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T233160 NZ_CP128548:c3473980-3473687 [Pseudomonas kurunegalensis]
GTGCCACGACTGATCGTCACGGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCGCGCCGTGCTGCACAGGTAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATTGAGTTTGGTGACTCAGGTTACGTCGCGTTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT233160 WP_004026357.1 NZ_CP091327:197856-198299 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT233160 NZ_CP128548:c3474240-3473968 [Pseudomonas kurunegalensis]
ATGGCCACTTCCATCAAAATTGATGACAAGCTAAAAGGCCGCATTCAGCAGTTGGCGAGCCAACGCCAGCGCTCGTCTCA
CTGGATCATGCGCGAGGCAATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTTAAGCAGGAAGCCCTGGCAT
CGTGGGCGGCATATCAGGAAACCGGACGGCATTTGACCAGCCAAGAAACTCGGACCTGGCTCGGTAGCTGGGGAACAGAT
ACTGAGGCAGAGTTGCCCAAGTGCCACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References