Detailed information of TA system TA233159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 85910..86580
• Replicon: plasmid phvK2115
• Accession: NZ_CP091327
• Organism: Klebsiella pneumoniae strain hvK2115

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: L3Y29_RS25950
• Protein ID: WP_004213072.1
• Coordinates: 85910..86353 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: L3Y29_RS25955
• Protein ID: WP_004213073.1
• Coordinates: 86350..86580 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Y29_RS25915 (L3Y29_25915)	81318..81593	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
L3Y29_RS25920 (L3Y29_25920)	81656..82147	+	492	WP_011251327.1	DM13 domain-containing protein	-
L3Y29_RS25925 (L3Y29_25925)	82196..83116	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
L3Y29_RS25930 (L3Y29_25930)	83207..83610	+	404	Protein_94	GAF domain-containing protein	-
L3Y29_RS25935 (L3Y29_25935)	84129..84765	-	637	Protein_95	mucoid phenotype regulator RmpA2	-
L3Y29_RS25940 (L3Y29_25940)	85183..85487	+	305	Protein_96	transposase	-
L3Y29_RS25945 (L3Y29_25945)	85510..85761	-	252	WP_186987481.1	hypothetical protein	-
L3Y29_RS25950 (L3Y29_25950)	85910..86353	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L3Y29_RS25955 (L3Y29_25955)	86350..86580	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L3Y29_RS25960 (L3Y29_25960)	87188..88321	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
L3Y29_RS25965 (L3Y29_25965)	88337..88630	+	294	WP_004213076.1	hypothetical protein	-
L3Y29_RS25970 (L3Y29_25970)	88620..88826	-	207	WP_004213077.1	hypothetical protein	-
L3Y29_RS25975 (L3Y29_25975)	89178..89468	+	291	WP_004213078.1	hypothetical protein	-
L3Y29_RS25980 (L3Y29_25980)	89458..90357	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / rmpA / iroN / iroD / iroC / iroB	1..223967	223967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T233159 WP_004213072.1 NZ_CP091327:c86353-85910 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T233159 NZ_CP128548:c343761-343414 [Pseudomonas kurunegalensis]
ATGAGTAAGCCGACCATACGCTTCACCCATTCCGCCTCTCAGAGCATCGAAGATCAAGTCCACCATCTGGCCGTCTATCA
CGGTACCGCAGCGGCACTTGAGAAAATCACCACACTGGTCGACACCATTGAGCACCGGCTGGCTGCCGCGCCCGTGGGTT
ACCCGGTCAGCCCACAAGCGAGCGGGCTGGGTGTCATGCAGTATCGTGAGCTGAATGTCGACGGGTACAGGGTGTTCTAC
GAAGTGATCGAAACCGACAATGCCATTGCCGTGGGGTTGGTTCTCAGGCAAAAGCAGAGTGTTGAACAGGCATTGATTCG
GTATTGCCTGATTTACCCATTACCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT233159 WP_004213073.1 NZ_CP091327:c86580-86350 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT233159 NZ_CP128548:c344020-343772 [Pseudomonas kurunegalensis]
ATGCGAGTAGAAACGATCAGCTACCTGAAACGCCATGCTGCTGACCTGGACCTGGAAGAACCCATGGTCGTGACACAGAA
CGGTGTGCCTGCCTATGTGGTCGAGTCCTACGCGGATCGCAAGCAACGGGATGAAGCCATAGCCTTGGTGAAACTGCTCG
CCATCGGTTCCCGTGAGTATGCTCAGGGCAAACACAGCTCGGCCGAAGACCTCAAGACTCGCCTTCTCAAGAAATTTTCT
GAGGAGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620