Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53734..54377 Replicon plasmid phvK2115
Accession NZ_CP091327
Organism Klebsiella pneumoniae strain hvK2115

Toxin (Protein)


Gene name vagD Uniprot ID A0A3A3ZBE4
Locus tag L3Y29_RS25760 Protein ID WP_007374381.1
Coordinates 53734..54150 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID G8LQB1
Locus tag L3Y29_RS25765 Protein ID WP_007853351.1
Coordinates 54147..54377 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y29_RS25735 (L3Y29_25735) 49218..49556 - 339 WP_007374386.1 carboxymuconolactone decarboxylase family protein -
L3Y29_RS25740 (L3Y29_25740) 49573..50142 - 570 WP_065907716.1 TetR/AcrR family transcriptional regulator -
L3Y29_RS25745 (L3Y29_25745) 50326..50883 - 558 WP_065907718.1 OsmC family protein -
L3Y29_RS25750 (L3Y29_25750) 51092..52114 - 1023 WP_007374383.1 hypothetical protein -
L3Y29_RS25755 (L3Y29_25755) 52099..53661 - 1563 WP_042936981.1 AAA family ATPase -
L3Y29_RS25760 (L3Y29_25760) 53734..54150 - 417 WP_007374381.1 type II toxin-antitoxin system VapC family toxin Toxin
L3Y29_RS25765 (L3Y29_25765) 54147..54377 - 231 WP_007853351.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L3Y29_RS25770 (L3Y29_25770) 55307..56128 + 822 WP_004213562.1 hypothetical protein -
L3Y29_RS25775 (L3Y29_25775) 56125..56904 + 780 WP_004213560.1 site-specific integrase -
L3Y29_RS25780 (L3Y29_25780) 57049..57978 - 930 WP_004213558.1 Rpn family recombination-promoting nuclease/putative transposase -
L3Y29_RS25785 (L3Y29_25785) 58281..58448 + 168 Protein_65 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iucA / iucB / iucC / iucD / iutA / rmpA2 / rmpA / iroN / iroD / iroC / iroB 1..223967 223967
- flank IS/Tn - - 58563..59531 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15105.57 Da        Isoelectric Point: 7.8882

>T233158 WP_007374381.1 NZ_CP091327:c54150-53734 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYAEMRFGATGPKAAPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T233158 NZ_CP128547:5242944-5243411 [Pseudomonas monteilii]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
GGCACTCAGCGCTCAAGTGCAGGCTCAGATGCACAAGGACCCAACGGGTTACACGCGCAGAAATGCCTATAAAAGGCTGG
CGGCCATCAAGCGACTGGCCTTCGATGTAATCCCTCAAGACCCGACCAAACCTGAGTACCGGCAGGGGGCCACACTGGGT
GGTGACCACAAGCACTGGTTCAGGGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTATCACACCCCCAGCAGGAT
CATTGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTACGAAAGTAGCGACGATGCTTACAAGGTCTTTCAGA
AGATGCTGCATACCGGCCACCCGCCGGACGATTGGGAGCAGCTACTGCGAGAAGCTGCAGCCGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8674.77 Da        Isoelectric Point: 4.4692

>AT233158 WP_007853351.1 NZ_CP091327:c54377-54147 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRVNL

Download         Length: 231 bp

>AT233158 NZ_CP128547:5242621-5242947 [Pseudomonas monteilii]
ATGGCAACCACTCTCGACGTCGAGTCCACACTGACCGATCGCTATCAGACCACTGTTCCCGAAACAGTACGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCTGACGGTGAGGTGATACTCACCCGTGCAGAAGCAACCG
AGAGCGATCCAGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCCACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGACCTCATCGCGCGTCTCGACAAGCTTGTCGGCGACGCAGAGGTCGACCTGGATCAATCTCTGTCGGCGGATGA
TGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A3ZBE4

Antitoxin

Source ID Structure
AlphaFold DB A0A3A3Z894

References