Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4886461..4887271 Replicon chromosome
Accession NZ_CP091326
Organism Klebsiella pneumoniae strain hvK2115

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag L3Y29_RS23745 Protein ID WP_004178461.1
Coordinates 4886738..4887271 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag L3Y29_RS23740 Protein ID WP_004222378.1
Coordinates 4886461..4886727 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y29_RS23720 (4882333) 4882333..4882677 - 345 WP_004222368.1 cation efflux system protein CusF -
L3Y29_RS23725 (4882695) 4882695..4884080 - 1386 WP_004222371.1 efflux transporter outer membrane subunit -
L3Y29_RS23730 (4884252) 4884252..4884935 + 684 WP_159147203.1 copper response regulator transcription factor CusR -
L3Y29_RS23735 (4884925) 4884925..4886358 + 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
L3Y29_RS23740 (4886461) 4886461..4886727 + 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L3Y29_RS23745 (4886738) 4886738..4887271 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
L3Y29_RS23750 (4887319) 4887319..4888440 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T233157 WP_004178461.1 NZ_CP091326:4886738-4887271 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T233157 NZ_CP128547:c4761657-4761370 [Pseudomonas monteilii]
ATGACCTATAGCCTCGACTTCGATTGCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACAGTGCGTGAGCAGTTCAA
GAAGAAACTGGCAGAGGTCTTGCTCAATCCTCGAGTCGAGGCAAACCGCCTTCACTCGCTGCCAGATTGCTACAAAATCA
AGCTTCGCAGCAGTGGGTACAGGCTGGTCTATCAGGTCATTGGCCAAGAGATCGTGGTTTTTGTCGTTGCTGTTGATCGC
CGTGAACGGGACCAAGCTTACAAAAAGGCTGCTGAGCGGCTTGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT233157 WP_004222378.1 NZ_CP091326:4886461-4886727 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT233157 NZ_CP128547:c4761886-4761647 [Pseudomonas monteilii]
ATGGCTTCCATCAATATCCGCGTCGACGACGATCTCAAAGCTCGCGCCTACAAAGAGCTTGAGCGTCTTGGTGTAACCCC
CTCCGAACTCATGCGCCAAGCCCTGCAATATGTAGCTGAGCGGGGCAAGCTACCCTTCCGGCCGGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCCACTGTACAAGAGAGGCTTGCTTCCCCGCAGCGCGTGAGGGTTCAGCTGGATGACCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References