Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4273011..4273636 Replicon chromosome
Accession NZ_CP091326
Organism Klebsiella pneumoniae strain hvK2115

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L3Y29_RS20890 Protein ID WP_002882817.1
Coordinates 4273253..4273636 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L3Y29_RS20885 Protein ID WP_004150355.1
Coordinates 4273011..4273253 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y29_RS20860 (4269002) 4269002..4269601 + 600 WP_004901669.1 glucose-1-phosphatase -
L3Y29_RS20865 (4269595) 4269595..4270455 + 861 WP_002882807.1 virulence factor BrkB family protein -
L3Y29_RS20870 (4270452) 4270452..4270889 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L3Y29_RS20875 (4270934) 4270934..4271875 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L3Y29_RS20880 (4271889) 4271889..4272806 - 918 WP_029884108.1 alpha/beta hydrolase -
L3Y29_RS20885 (4273011) 4273011..4273253 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L3Y29_RS20890 (4273253) 4273253..4273636 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L3Y29_RS20895 (4273810) 4273810..4274739 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L3Y29_RS20900 (4274736) 4274736..4275371 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L3Y29_RS20905 (4275368) 4275368..4276270 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T233155 WP_002882817.1 NZ_CP091326:4273253-4273636 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T233155 NZ_CP128546:5158242-5158709 [Pseudomonas monteilii]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
GGCACTCAGCGCTCAAGTGCAGGCTCAGATGCACAAGGACCCAACGGGTTACACGCGCAGAAATGCCTATAAAAGGCTGG
CGGCCATCAAGCGACTGGCCTTCGATGTAATCCCTCAAGACCCGACCAAACCTGAGTACCGGCAGGGGGCCACACTGGGT
GGTGACCACAAGCACTGGTTCAGGGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTATCACACCCCCAGCAGGAT
CATTGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTACGAAAGTAGCGACGATGCTTACAAGGTCTTTCAGA
AGATGCTGCATACCGGCCACCCGCCGGACGATTGGGAGCAGCTACTGCGAGAAGCTGCAGCCGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT233155 WP_004150355.1 NZ_CP091326:4273011-4273253 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT233155 NZ_CP128546:5157919-5158245 [Pseudomonas monteilii]
ATGGCAACCACTCTCGACGTCGAGTCCACACTGACCGATCGCTATCAGACCACTGTTCCCGAAACAGTACGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCTGACGGTGAGGTGATACTCACCCGTGCAGAAGCAACCG
AGAGCGATCCAGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCCACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGACCTCATCGCGCGTCTCGACAAGCTTGTCGGCGACGCAGAGGTCGACCTGGATCAATCTCTGTCGGCGGATGA
TGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References