Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3993839..3994578 Replicon chromosome
Accession NZ_CP091326
Organism Klebsiella pneumoniae strain hvK2115

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag L3Y29_RS19545 Protein ID WP_004901283.1
Coordinates 3993839..3994324 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L3Y29_RS19550 Protein ID WP_003026799.1
Coordinates 3994312..3994578 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3Y29_RS19520 (3989468) 3989468..3989758 + 291 WP_002921931.1 HTH-type transcriptional regulator -
L3Y29_RS19525 (3990010) 3990010..3990222 + 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
L3Y29_RS19530 (3990321) 3990321..3991940 - 1620 WP_004901278.1 ATP-binding cassette domain-containing protein -
L3Y29_RS19535 (3992242) 3992242..3992394 - 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
L3Y29_RS19540 (3992468) 3992468..3993514 + 1047 WP_004173931.1 IS481-like element ISKpn28 family transposase -
L3Y29_RS19545 (3993839) 3993839..3994324 - 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
L3Y29_RS19550 (3994312) 3994312..3994578 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L3Y29_RS19555 (3994711) 3994711..3995139 - 429 WP_004901287.1 GFA family protein -
L3Y29_RS19565 (3997005) 3997005..3999074 - 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 3992468..3995972 3504


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T233154 WP_004901283.1 NZ_CP091326:c3994324-3993839 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T233154 NZ_CP128546:c4675618-4675331 [Pseudomonas monteilii]
ATGACCTATAGCCTCGACTTCGATTGCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACAGTGCGTGAGCAGTTCAA
GAAGAAACTGGCAGAGGTCTTGCTCAATCCTCGAGTCGAGGCAAACCGCCTTCACTCGCTGCCAGATTGCTACAAAATCA
AGCTTCGCAGCAGTGGGTACAGGCTGGTCTATCAGGTCATTGGCCAAGAGATCGTGGTTTTTGTCGTTGCTGTTGATCGC
CGTGAACGGGACCAAGCTTACAAAAAGGCTGCTGAGCGGCTTGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT233154 WP_003026799.1 NZ_CP091326:c3994578-3994312 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT233154 NZ_CP128546:c4675847-4675608 [Pseudomonas monteilii]
ATGGCTTCCATCAATATCCGCGTCGACGACGATCTCAAAGCTCGCGCCTACAAAGAGCTTGAGCGTCTTGGTGTAACCCC
CTCCGAACTCATGCGCCAAGCCCTGCAATATGTAGCTGAGCGGGGCAAGCTACCCTTCCGGCCGGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCCACTGTACAAGAGAGGCTTGCTTCCCCGCAGCGCGTGAGGGTTCAGCTGGATGACCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References