Detailed information of TA system TA233142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1540143..1541731
• Replicon: chromosome
• Accession: NZ_CP091326
• Organism: Klebsiella pneumoniae strain hvK2115

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: L3Y29_RS07540
• Protein ID: WP_002904792.1
• Coordinates: 1540409..1541731 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: L3Y29_RS07535
• Protein ID: WP_004176198.1
• Coordinates: 1540143..1540409 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3Y29_RS07510 (1535172)	1535172..1536095	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
L3Y29_RS07515 (1536076)	1536076..1537230	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
L3Y29_RS07520 (1537576)	1537576..1538955	+	1380	WP_014599164.1	nucleobase:cation symporter-2 family protein	-
L3Y29_RS07525 (1538933)	1538933..1539433	+	501	WP_004224756.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
L3Y29_RS07530 (1539430)	1539430..1539756	+	327	WP_004224757.1	hydroxyisourate hydrolase	-
L3Y29_RS07535 (1540143)	1540143..1540409	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
L3Y29_RS07540 (1540409)	1540409..1541731	+	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
L3Y29_RS07545 (1541748)	1541748..1543040	-	1293	WP_016946381.1	glycoside hydrolase family 10 protein	-
L3Y29_RS07550 (1543182)	1543182..1544186	-	1005	WP_029884185.1	fimbrial protein	-
L3Y29_RS07555 (1544174)	1544174..1546675	-	2502	WP_029884184.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T233142 WP_002904792.1 NZ_CP091326:1540409-1541731 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T233142 NZ_CP128542:4018151-4018726 [Pseudomonas asiatica]
ATGAAGCACTCGCCCTTCACGGCCATTTACGACGCGAACGTTCTTTATCCTGCCCCACTCCGTGATTTCCTGATGAACCT
TGCATTGACAGGTATTTACCGAGCACGTTGGTCAACGAAGATTCATGACGAGTGGAAACGCAATTTATTGCTCAACCGGC
CTGACCTCACCCTCGAGCAAGTAGATCGGACGTCACGGCGAATGGACGCTGCTGTACCTGACGCACTCGTAACTGACTAC
GAGTCGCTGGTAGAAGGGCTGGACTTGCCAGATAAGGATGATCGACATGTCCTCGCTGCTGCAATCAAGTGCAACGCTTC
CGTCATCGTCACCTTCAACCTGAAGGATTTCCCCGAAACGGCTCTCGACGTTTTCGATATCGAGCCCTTACACCCGGATG
ACTTCATCGCCGACCTGTGGGACCTGGACAAGGCAGCAGTACTTGAGGCTGCCCAGCGGCAGCGAATGTCCTTGAAAAAC
CCACCTCACAGCGTGCAGCAGTATCTCGACAGACTGCTTCAGCAAAAGCTTCCCGAGTCGGTGAAGTTGCTTTCAGAATT
CAAATTCCTCCTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT233142 WP_004176198.1 NZ_CP091326:1540143-1540409 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT233142 NZ_CP128542:4017687-4018154 [Pseudomonas asiatica]
ATGACCACCGCCGACCTCTCTCGCAAGATGCTTCCCGATGAGAAAGAGATTGCCGTGGCAGTCGAATCGAGCCGAACGCT
AGCAGCCTTCCTCTCGACAAAATCAGATACCCAACGGATTGAGCTGCTCAATGAAGAGAATCAGCGCCAGGTTGTTGAAG
TACCTACCTTCGCGCTGCGTCTGTTTGGCGAGATTCTCAGCGAGCTGGCTCTGGGGAACTCGGTCAAAGTCGTTCCAATC
CACGCCGAATTGACTACCCAAGAGGCAGCAGACCTACTCAACGTCTCTCGGCCGTACTTGGTAAAACTGCTAGACGAATC
TGTGATTCCTCACACAAAAACGGGTCGCCATCGTCGGGTCAAATTCTCCGACCTGGTTGCCTACAAGGACAAACGTGACG
AGGTAAGCCGTGCCGCAATGGACGCGCTGGCAGCCGAAGCCCAGGAGTTACGGATGGGGTACGAATGA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8