Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 220833..221359 Replicon plasmid pDR25-1
Accession NZ_CP091320
Organism Enterobacter sp. JH25

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag L2Y28_RS23730 Protein ID WP_000323025.1
Coordinates 220833..221120 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag L2Y28_RS23735 Protein ID WP_000534858.1
Coordinates 221120..221359 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y28_RS23695 216403..216579 - 177 WP_001371930.1 hypothetical protein -
L2Y28_RS23700 217090..218034 + 945 WP_236919602.1 DUF5417 domain-containing protein -
L2Y28_RS23705 218130..218732 + 603 WP_001371889.1 hypothetical protein -
L2Y28_RS23710 218792..219142 + 351 WP_000743059.1 hypothetical protein -
L2Y28_RS23715 219189..219392 + 204 WP_001015183.1 hypothetical protein -
L2Y28_RS23720 219674..219994 + 321 WP_000332796.1 hypothetical protein -
L2Y28_RS23725 220607..220732 - 126 WP_229020258.1 DUF5431 family protein -
L2Y28_RS23730 220833..221120 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
L2Y28_RS23735 221120..221359 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
L2Y28_RS23740 221384..221488 + 105 Protein_228 hypothetical protein -
L2Y28_RS23745 221622..222545 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
L2Y28_RS23750 222745..223317 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
L2Y28_RS23755 223793..225031 - 1239 WP_236919603.1 IS110-like element ISEsa2 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / tet(A) / mcr-9 - 1..275976 275976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T233139 WP_000323025.1 NZ_CP091320:c221120-220833 [Enterobacter sp. JH25]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T233139 NZ_CP128542:994560-994856 [Pseudomonas asiatica]
ATGAGCGAAAAGTTGATCCCGTTTGATATGGCGGCCTTGTTGAGTAGTGATGAGGCCATCACTGAGTACCTTAGTCAGGT
CCTCGCAGATGGCGATACCGAGGAAATAATTCGGGCACTTGGCCATATCGCTCGCGCACGGGGAATGACGCGTATCGCCA
CCGAGAGTGGGTTGGGAAGGGAAAGTCTGTACAAGGCACTCAGTCCAGGCGCAAAACCACGCTTTGACACTGTGCTGAAA
GTCATTCGTGCCTTGGGTGTCGACCTCCTCGTTCAGCCCCATGTGGTTCCCCGCTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT233139 WP_000534858.1 NZ_CP091320:c221359-221120 [Enterobacter sp. JH25]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT233139 NZ_CP128542:994270-994563 [Pseudomonas asiatica]
ATGTATCTCGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGAGATTTGAGGGCCCAGCTTGCGATAGG
TCGTAGATTGGAGCGAGCAGCAATGGGGAATCTTGGAGATGTCAAATGGCTTGGAGGCGGTATCGGTGAGCTTCGCATCG
ATGTAGCTGCTGGATATCGTGTCTATTTTGCGCAAAAGGGCCAGAGGCTGGTTCTGCTGTTGGTCGGTGGAGACAAGTCC
ACCCAGGCCACCGATATCCTCAAAGCCAGAAAACTGCTGAAGGAGATGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References