Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93826..95449 Replicon plasmid pDR25-1
Accession NZ_CP091320
Organism Enterobacter sp. JH25

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag L2Y28_RS23050 Protein ID WP_000088044.1
Coordinates 94124..95449 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag L2Y28_RS23045 Protein ID WP_012477377.1
Coordinates 93826..94119 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y28_RS23025 89625..90017 + 393 WP_000341066.1 hypothetical protein -
L2Y28_RS23030 90676..91332 + 657 WP_000462754.1 trypsin-like serine protease -
L2Y28_RS23035 91535..92032 + 498 WP_000340139.1 membrane protein -
L2Y28_RS23040 92037..93425 + 1389 WP_012695445.1 hypothetical protein -
L2Y28_RS23045 93826..94119 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
L2Y28_RS23050 94124..95449 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
L2Y28_RS23055 95510..95716 + 207 WP_000134171.1 hypothetical protein -
L2Y28_RS23060 95817..96227 + 411 WP_000985909.1 hypothetical protein -
L2Y28_RS23065 96240..96764 + 525 WP_001805097.1 hypothetical protein -
L2Y28_RS23070 96946..97950 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / tet(A) / mcr-9 - 1..275976 275976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T233138 WP_000088044.1 NZ_CP091320:94124-95449 [Enterobacter sp. JH25]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T233138 NZ_CP128542:994270-994563 [Pseudomonas asiatica]
ATGTATCTCGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGAGATTTGAGGGCCCAGCTTGCGATAGG
TCGTAGATTGGAGCGAGCAGCAATGGGGAATCTTGGAGATGTCAAATGGCTTGGAGGCGGTATCGGTGAGCTTCGCATCG
ATGTAGCTGCTGGATATCGTGTCTATTTTGCGCAAAAGGGCCAGAGGCTGGTTCTGCTGTTGGTCGGTGGAGACAAGTCC
ACCCAGGCCACCGATATCCTCAAAGCCAGAAAACTGCTGAAGGAGATGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT233138 WP_012477377.1 NZ_CP091320:93826-94119 [Enterobacter sp. JH25]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT233138 NZ_CP128542:994560-994856 [Pseudomonas asiatica]
ATGAGCGAAAAGTTGATCCCGTTTGATATGGCGGCCTTGTTGAGTAGTGATGAGGCCATCACTGAGTACCTTAGTCAGGT
CCTCGCAGATGGCGATACCGAGGAAATAATTCGGGCACTTGGCCATATCGCTCGCGCACGGGGAATGACGCGTATCGCCA
CCGAGAGTGGGTTGGGAAGGGAAAGTCTGTACAAGGCACTCAGTCCAGGCGCAAAACCACGCTTTGACACTGTGCTGAAA
GTCATTCGTGCCTTGGGTGTCGACCTCCTCGTTCAGCCCCATGTGGTTCCCCGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References