Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3830072..3830853 Replicon chromosome
Accession NZ_CP091319
Organism Enterobacter sp. JH25

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A431SHU0
Locus tag L2Y28_RS18360 Protein ID WP_022649378.1
Coordinates 3830072..3830563 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A3S0FY21
Locus tag L2Y28_RS18365 Protein ID WP_022649379.1
Coordinates 3830560..3830853 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y28_RS18345 3825352..3825804 - 453 WP_022649375.1 hypothetical protein -
L2Y28_RS18350 3826319..3828229 + 1911 WP_022649376.1 hypothetical protein -
L2Y28_RS18355 3828226..3829920 + 1695 WP_022649377.1 hypothetical protein -
L2Y28_RS18360 3830072..3830563 - 492 WP_022649378.1 GNAT family N-acetyltransferase Toxin
L2Y28_RS18365 3830560..3830853 - 294 WP_022649379.1 DUF1778 domain-containing protein Antitoxin
L2Y28_RS18370 3830955..3832748 - 1794 WP_022649380.1 hybrid sensor histidine kinase/response regulator -
L2Y28_RS18375 3832745..3833377 - 633 WP_022649381.1 response regulator transcription factor -
L2Y28_RS18380 3833553..3833867 + 315 WP_003862457.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3825352..3833867 8515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(83-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17469.36 Da        Isoelectric Point: 8.1405

>T233136 WP_022649378.1 NZ_CP091319:c3830563-3830072 [Enterobacter sp. JH25]
MISAPVPLRAGHILTPFCCGVDSMDNWLKQRAMKNQASGASRTFVCCGGDSNVLAYYALASSAVTTNTVPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAESIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLV
ASI

Download         Length: 492 bp

>T233136 NZ_CP128541:c4867740-4867453 [Pseudomonas monteilii]
ATGACCTATAGCCTCGACTTCGATTGCCGCGCATTGAAAGAGTGGAAGAAGCTGGGCGATACAGTGCGTGAGCAGTTCAA
GAAGAAACTGGCAGAGGTCTTGCTCAATCCTCGAGTCGAGGCAAACCGCCTTCACTCGCTGCCAGATTGCTACAAAATCA
AGCTTCGCAGCAGTGGGTACAGGCTGGTCTATCAGGTCATTGGCCAAGAGATCGTGGTTTTTGTCGTTGCTGTTGATCGC
CGTGAACGGGACCAAGCTTACAAAAAGGCTGCTGAGCGGCTTGAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10959.58 Da        Isoelectric Point: 8.6351

>AT233136 WP_022649379.1 NZ_CP091319:c3830853-3830560 [Enterobacter sp. JH25]
MPAANTMAVKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLTRLDQAPAPN
AALRKTMQTLAPWEQEK

Download         Length: 294 bp

>AT233136 NZ_CP128541:c4867969-4867730 [Pseudomonas monteilii]
ATGGCTTCCATCAATATCCGCGTCGACGACGATCTCAAAGCTCGCGCCTACAAAGAGCTTGAGCGTCTTGGTGTAACCCC
CTCCGAACTCATGCGCCAAGCCCTGCAATATGTAGCTGAGCGGGGCAAGCTACCCTTCCGGCCGGTCCTGATGACCGAGG
AAGATGAAGACCTCATTGCCACTGTACAAGAGAGGCTTGCTTCCCCGCAGCGCGTGAGGGTTCAGCTGGATGACCTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SHU0

Antitoxin

Source ID Structure
AlphaFold DB A0A3S0FY21

References