Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2258100..2258839 Replicon chromosome
Accession NZ_CP091319
Organism Enterobacter sp. JH25

Toxin (Protein)


Gene name tacT Uniprot ID A0A3S0FZ61
Locus tag L2Y28_RS10920 Protein ID WP_022648141.1
Coordinates 2258100..2258585 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag L2Y28_RS10925 Protein ID WP_003857131.1
Coordinates 2258573..2258839 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y28_RS10895 2254256..2255278 + 1023 WP_022648135.1 alpha/beta hydrolase -
L2Y28_RS10900 2255374..2256138 + 765 WP_022648136.1 SDR family oxidoreductase -
L2Y28_RS10905 2256805..2257068 + 264 WP_022648138.1 DUF4225 domain-containing protein -
L2Y28_RS10910 2257043..2257372 - 330 WP_022648139.1 hypothetical protein -
L2Y28_RS10915 2257505..2258074 + 570 WP_022648140.1 hypothetical protein -
L2Y28_RS10920 2258100..2258585 - 486 WP_022648141.1 GNAT family N-acetyltransferase Toxin
L2Y28_RS10925 2258573..2258839 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
L2Y28_RS10930 2258903..2259832 - 930 WP_022648142.1 LysR family transcriptional regulator -
L2Y28_RS10935 2259962..2261350 + 1389 WP_022648143.1 MFS transporter -
L2Y28_RS10940 2261329..2261886 - 558 WP_022648144.1 OmpH family outer membrane protein -
L2Y28_RS10945 2262007..2263143 - 1137 WP_022648145.1 type 1 fimbrial protein -
L2Y28_RS10950 2263167..2263745 - 579 WP_022648146.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2235233..2261886 26653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17558.24 Da        Isoelectric Point: 9.6275

>T233125 WP_022648141.1 NZ_CP091319:c2258585-2258100 [Enterobacter sp. JH25]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T233125 NZ_CP128532:c1828277-1827981 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT233125 WP_003857131.1 NZ_CP091319:c2258839-2258573 [Enterobacter sp. JH25]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT233125 NZ_CP128532:c1827997-1827755 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S0FZ61

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References