Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1061460..1062081 Replicon chromosome
Accession NZ_CP091319
Organism Enterobacter sp. JH25

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag L2Y28_RS05035 Protein ID WP_015571250.1
Coordinates 1061460..1061678 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag L2Y28_RS05040 Protein ID WP_006809850.1
Coordinates 1061707..1062081 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Y28_RS05005 1057472..1057732 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
L2Y28_RS05010 1057735..1057875 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L2Y28_RS05015 1057872..1058582 - 711 WP_022647267.1 GNAT family protein -
L2Y28_RS05020 1058685..1060145 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
L2Y28_RS05025 1060117..1060584 - 468 WP_022647269.1 YlaC family protein -
L2Y28_RS05030 1060701..1061252 - 552 WP_022647270.1 maltose O-acetyltransferase -
L2Y28_RS05035 1061460..1061678 - 219 WP_015571250.1 HHA domain-containing protein Toxin
L2Y28_RS05040 1061707..1062081 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
L2Y28_RS05045 1062591..1065737 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
L2Y28_RS05050 1065760..1066953 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T233124 WP_015571250.1 NZ_CP091319:c1061678-1061460 [Enterobacter sp. JH25]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T233124 NZ_CP128532:c971709-971440 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT233124 WP_006809850.1 NZ_CP091319:c1062081-1061707 [Enterobacter sp. JH25]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT233124 NZ_CP128532:c971979-971713 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References