Detailed information of TA system TA233108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 211463..212118
• Replicon: chromosome
• Accession: NZ_CP091308
• Organism: Burkholderia pyrrocinia strain LWK2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L2Y90_RS18340
• Protein ID: WP_258284091.1
• Coordinates: 211463..211882 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S8IH63
• Locus tag: L2Y90_RS18345
• Protein ID: WP_034180974.1
• Coordinates: 211879..212118 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y90_RS18320 (L2Y90_18275)	206629..207201	+	573	WP_034180472.1	hypothetical protein	-
L2Y90_RS18325 (L2Y90_18280)	208198..209337	-	1140	WP_258284090.1	hypothetical protein	-
L2Y90_RS18330 (L2Y90_18285)	209668..209802	-	135	WP_021158165.1	entericidin A/B family lipoprotein	-
L2Y90_RS18335 (L2Y90_18290)	210036..211331	+	1296	WP_034180473.1	aspartate carbamoyltransferase	-
L2Y90_RS18340 (L2Y90_18295)	211463..211882	-	420	WP_258284091.1	type II toxin-antitoxin system VapC family toxin	Toxin
L2Y90_RS18345 (L2Y90_18300)	211879..212118	-	240	WP_034180974.1	Arc family DNA-binding protein	Antitoxin
L2Y90_RS18350 (L2Y90_18305)	212430..214664	+	2235	WP_258284092.1	TonB-dependent siderophore receptor	-
L2Y90_RS18355 (L2Y90_18310)	214668..215423	+	756	WP_258284093.1	tetratricopeptide repeat protein	-
L2Y90_RS18360 (L2Y90_18315)	215420..216103	+	684	WP_258284094.1	Fe2+-dependent dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15277.70 Da
• Isoelectric Point: 6.9861

>T233108 WP_258284091.1 NZ_CP091308:c211882-211463 [Burkholderia pyrrocinia]
MILVDTNVISEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAVLPEGRRREWLHQSIERRVVPLFRGRILPFDD
AASKAYASLRARARVAGSAIAPVDCFIAATAEANGLIVATRDVAPFEAVGLRVIDPWAR

Nucleotide

• Length: 420 bp

>T233108 NZ_CP128530:700182-700478 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8707.89 Da
• Isoelectric Point: 5.6864

>AT233108 WP_034180974.1 NZ_CP091308:c212118-211879 [Burkholderia pyrrocinia]
MPVITVRNLPDEVHRALRIRAAQHGRSTEAEVRDILEQAVLPGGRLKLGTLLAEIGREAGGVDFDVQRDKTSTDPMSFE

Nucleotide

• Length: 240 bp

>AT233108 NZ_CP128530:700462-700704 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A2S8IH63