Detailed information of TA system TA233103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 41982..43090
• Replicon: plasmid pUK043_2
• Accession: NZ_CP091303
• Organism: Enterococcus faecium strain UK043

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A0M4MHJ8
• Locus tag: L3K57_RS15055
• Protein ID: WP_002303392.1
• Coordinates: 41982..42851 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: B9DSK1
• Locus tag: L3K57_RS15060
• Protein ID: WP_002303393.1
• Coordinates: 42866..43090 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3K57_RS15025 (L3K57_15025)	37278..37529	-	252	Protein_47	GNAT family N-acetyltransferase	-
L3K57_RS15030 (L3K57_15030)	37774..39069	+	1296	WP_002297218.1	ISL3-like element ISEfa11 family transposase	-
L3K57_RS15035 (L3K57_15035)	39170..40078	+	909	WP_002324522.1	IS982-like element ISEfm1 family transposase	-
L3K57_RS15040 (L3K57_15040)	40102..40329	-	228	Protein_50	hypothetical protein	-
L3K57_RS15045 (L3K57_15045)	40326..41234	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
L3K57_RS15050 (L3K57_15050)	41267..42001	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
L3K57_RS15055 (L3K57_15055)	41982..42851	-	870	WP_002303392.1	nucleotidyltransferase domain-containing protein	Toxin
L3K57_RS15060 (L3K57_15060)	42866..43090	-	225	WP_002303393.1	helix-turn-helix transcriptional regulator	Antitoxin
L3K57_RS15065 (L3K57_15065)	43233..43334	-	102	Protein_55	site-specific recombinase	-
L3K57_RS15070 (L3K57_15070)	43476..44750	+	1275	WP_002398627.1	ISL3-like element IS1476 family transposase	-
L3K57_RS15075 (L3K57_15075)	44838..45563	-	726	Protein_57	recombinase zinc beta ribbon domain-containing protein	-
L3K57_RS15080 (L3K57_15080)	46251..47054	-	804	Protein_58	lincosamide nucleotidyltransferase Lnu(B)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32807.38 Da
• Isoelectric Point: 4.7269

>T233103 WP_002303392.1 NZ_CP091303:c42851-41982 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T233103 NZ_CP128529:c2052295-2052026 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT233103 WP_002303393.1 NZ_CP091303:c43090-42866 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT233103 NZ_CP128529:c2052548-2052282 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Structures

Toxin

• AlphaFold DB: A0A0M4MHJ8

Antitoxin

• AlphaFold DB: A0A1M5SHM7