Detailed information of TA system TA233102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 8548..9656
• Replicon: plasmid pUK043_2
• Accession: NZ_CP091303
• Organism: Enterococcus faecium strain UK043

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A0M4MHJ8
• Locus tag: L3K57_RS14850
• Protein ID: WP_002303392.1
• Coordinates: 8548..9417 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: B9DSK1
• Locus tag: L3K57_RS14855
• Protein ID: WP_002303393.1
• Coordinates: 9432..9656 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3K57_RS14815 (L3K57_14815)	3781..4032	-	252	Protein_6	GNAT family N-acetyltransferase	-
L3K57_RS14820 (L3K57_14820)	4277..5572	+	1296	WP_236918137.1	ISL3-like element ISEfa11 family transposase	-
L3K57_RS14825 (L3K57_14825)	5673..6580	+	908	WP_236918138.1	IS982-like element ISEfm1 family transposase	-
L3K57_RS14830 (L3K57_14830)	6604..6834	-	231	Protein_9	hypothetical protein	-
L3K57_RS14835 (L3K57_14835)	6831..7807	-	977	Protein_10	aminoglycoside 6-adenylyltransferase	-
L3K57_RS14845 (L3K57_14845)	7862..8567	-	706	Protein_11	class I SAM-dependent methyltransferase	-
L3K57_RS14850 (L3K57_14850)	8548..9417	-	870	WP_002303392.1	nucleotidyltransferase domain-containing protein	Toxin
L3K57_RS14855 (L3K57_14855)	9432..9656	-	225	WP_002303393.1	helix-turn-helix transcriptional regulator	Antitoxin
L3K57_RS14860 (L3K57_14860)	9799..9900	-	102	Protein_14	site-specific recombinase	-
L3K57_RS14865 (L3K57_14865)	10042..11316	+	1275	WP_002398627.1	ISL3-like element IS1476 family transposase	-
L3K57_RS14870 (L3K57_14870)	11404..12129	-	726	Protein_16	recombinase zinc beta ribbon domain-containing protein	-
L3K57_RS14875 (L3K57_14875)	12817..13620	-	804	Protein_17	lincosamide nucleotidyltransferase Lnu(B)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32807.38 Da
• Isoelectric Point: 4.7269

>T233102 WP_002303392.1 NZ_CP091303:c9417-8548 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T233102 NZ_CP128529:1541959-1542183 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT233102 WP_002303393.1 NZ_CP091303:c9656-9432 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT233102 NZ_CP128529:1542226-1542555 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0M4MHJ8

Antitoxin

• AlphaFold DB: A0A1M5SHM7