Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | omega-epsilon-zeta/zeta-epsilon |
| Location | 33570..34707 | Replicon | plasmid pUK043_1 |
| Accession | NZ_CP091302 | ||
| Organism | Enterococcus faecium strain UK043 | ||
Toxin (Protein)
| Gene name | zeta | Uniprot ID | Q54944 |
| Locus tag | L3K57_RS14130 | Protein ID | WP_001284311.1 |
| Coordinates | 33570..34433 (-) | Length | 288 a.a. |
Antitoxin (Protein)
| Gene name | epsilon | Uniprot ID | R2XCR7 |
| Locus tag | L3K57_RS14135 | Protein ID | WP_000301765.1 |
| Coordinates | 34435..34707 (-) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L3K57_RS14095 (L3K57_14095) | 28676..29056 | - | 381 | Protein_31 | IS6-like element IS1216 family transposase | - |
| L3K57_RS14100 (L3K57_14100) | 29126..29806 | + | 681 | WP_002319817.1 | IS6-like element ISS1N family transposase | - |
| L3K57_RS14105 (L3K57_14105) | 30150..30404 | + | 255 | Protein_33 | replication initiation protein | - |
| L3K57_RS14110 (L3K57_14110) | 30474..30986 | + | 513 | WP_002387940.1 | transposase | - |
| L3K57_RS14115 (L3K57_14115) | 30983..32020 | + | 1038 | WP_002303110.1 | IS3 family transposase | - |
| L3K57_RS14120 (L3K57_14120) | 32097..32777 | - | 681 | WP_001015311.1 | IS6-like element IS1216 family transposase | - |
| L3K57_RS14125 (L3K57_14125) | 32852..33307 | + | 456 | Protein_37 | rRNA adenine N-6-methyltransferase family protein | - |
| L3K57_RS14130 (L3K57_14130) | 33570..34433 | - | 864 | WP_001284311.1 | zeta toxin family protein | Toxin |
| L3K57_RS14135 (L3K57_14135) | 34435..34707 | - | 273 | WP_000301765.1 | antitoxin | Antitoxin |
| L3K57_RS14140 (L3K57_14140) | 34724..34939 | - | 216 | WP_001835296.1 | peptide-binding protein | - |
| L3K57_RS14145 (L3K57_14145) | 35031..35288 | - | 258 | Protein_41 | chromosome partitioning protein ParA | - |
| L3K57_RS14150 (L3K57_14150) | 35344..36024 | - | 681 | WP_001015311.1 | IS6-like element IS1216 family transposase | - |
| L3K57_RS14155 (L3K57_14155) | 36244..36420 | + | 177 | Protein_43 | IS6 family transposase | - |
| L3K57_RS14160 (L3K57_14160) | 36497..38045 | - | 1549 | Protein_44 | IS66 family transposase | - |
| L3K57_RS14165 (L3K57_14165) | 38147..38500 | - | 354 | WP_002287659.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| L3K57_RS14170 (L3K57_14170) | 38490..38684 | - | 195 | WP_002285758.1 | hypothetical protein | - |
| L3K57_RS14175 (L3K57_14175) | 38767..39252 | - | 486 | Protein_47 | IS256 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..161029 | 161029 | |
| - | inside | IScluster/Tn | - | - | 26882..48963 | 22081 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 288 a.a. Molecular weight: 32404.02 Da Isoelectric Point: 6.9964
>T233100 WP_001284311.1 NZ_CP091302:c34433-33570 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI
Download Length: 864 bp
>T233100 NZ_CP128529:1098129-1098305 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10721.25 Da Isoelectric Point: 6.4676
>AT233100 WP_000301765.1 NZ_CP091302:c34707-34435 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
Download Length: 273 bp
>AT233100 NZ_CP128529:1098361-1098777 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 1GVN | |
| PDB | 3Q8X |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F0A3 |