Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2463958..2464529 Replicon chromosome
Accession NZ_CP091301
Organism Enterococcus faecium strain UK043

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag L3K57_RS12185 Protein ID WP_002286801.1
Coordinates 2464188..2464529 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag L3K57_RS12180 Protein ID WP_002323011.1
Coordinates 2463958..2464188 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3K57_RS12160 (L3K57_12160) 2459725..2461020 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
L3K57_RS12165 (L3K57_12165) 2461253..2461834 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
L3K57_RS12170 (L3K57_12170) 2462566..2463128 + 563 Protein_2344 SOS response-associated peptidase family protein -
L3K57_RS12175 (L3K57_12175) 2463333..2463671 - 339 WP_002286804.1 hypothetical protein -
L3K57_RS12180 (L3K57_12180) 2463958..2464188 + 231 WP_002323011.1 hypothetical protein Antitoxin
L3K57_RS12185 (L3K57_12185) 2464188..2464529 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L3K57_RS12190 (L3K57_12190) 2465379..2465564 + 186 WP_002304207.1 hypothetical protein -
L3K57_RS12195 (L3K57_12195) 2466069..2466263 + 195 WP_002297028.1 hypothetical protein -
L3K57_RS12200 (L3K57_12200) 2466454..2466703 + 250 Protein_2350 transposase -
L3K57_RS12205 (L3K57_12205) 2466947..2467777 - 831 WP_002286789.1 manganese catalase family protein -
L3K57_RS12210 (L3K57_12210) 2467985..2469319 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2459725..2461020 1295


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T233099 WP_002286801.1 NZ_CP091301:2464188-2464529 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T233099 NZ_CP128529:c781822-781640 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT233099 WP_002323011.1 NZ_CP091301:2463958-2464188 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT233099 NZ_CP128529:c781601-781182 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References