Detailed information of TA system TA233089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1587955..1588575
• Replicon: chromosome
• Accession: NZ_CP091300
• Organism: Salmonella enterica subsp. enterica serovar Bovismorbificans strain SB699

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: L1971_RS08715
• Protein ID: WP_001280991.1
• Coordinates: 1588357..1588575 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: L1971_RS08710
• Protein ID: WP_000344807.1
• Coordinates: 1587955..1588329 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1971_RS08700 (1583094)	1583094..1584287	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
L1971_RS08705 (1584310)	1584310..1587459	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
L1971_RS08710 (1587955)	1587955..1588329	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
L1971_RS08715 (1588357)	1588357..1588575	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
L1971_RS08720 (1588754)	1588754..1589305	+	552	WP_001278792.1	maltose O-acetyltransferase	-
L1971_RS08725 (1589423)	1589423..1589893	+	471	WP_000136183.1	YlaC family protein	-
L1971_RS08730 (1589949)	1589949..1590089	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
L1971_RS08735 (1590095)	1590095..1590355	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
L1971_RS08740 (1590580)	1590580..1592130	+	1551	WP_024792601.1	EAL domain-containing protein	-
L1971_RS08750 (1592361)	1592361..1592750	+	390	WP_000961285.1	MGMT family protein	-
L1971_RS08755 (1592783)	1592783..1593352	-	570	WP_000779802.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T233089 WP_001280991.1 NZ_CP091300:1588357-1588575 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T233089 NZ_CP128528:565507-565803 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT233089 WP_000344807.1 NZ_CP091300:1587955-1588329 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT233089 NZ_CP128528:565787-566029 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0