Detailed information of TA system TA233077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4969..5612
• Replicon: plasmid pSCD1
• Accession: NZ_CP091299
• Organism: Salmonella enterica subsp. enterica serovar Bovismorbificans strain SB699

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A8F2ZUT5
• Locus tag: L1971_RS00030
• Protein ID: WP_001697179.1
• Coordinates: 4969..5385 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A8F2UWJ1
• Locus tag: L1971_RS00035
• Protein ID: WP_001261289.1
• Coordinates: 5382..5612 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1971_RS00010 (L1971_00010)	625..1065	-	441	WP_237123633.1	hypothetical protein	-
L1971_RS00015 (L1971_00015)	1031..2866	-	1836	WP_237123636.1	P-loop NTPase fold protein	-
L1971_RS00020 (L1971_00020)	2868..3956	-	1089	WP_021003379.1	transcriptional repressor PifC	-
L1971_RS00025 (L1971_00025)	4194..4937	-	744	WP_001292424.1	hypothetical protein	-
L1971_RS00030 (L1971_00030)	4969..5385	-	417	WP_001697179.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1971_RS00035 (L1971_00035)	5382..5612	-	231	WP_001261289.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L1971_RS00040 (L1971_00040)	6356..6574	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	-
L1971_RS00045 (L1971_00045)	6576..6881	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	-
L1971_RS00050 (L1971_00050)	6883..7173	+	291	WP_001266176.1	hypothetical protein	-
L1971_RS00055 (L1971_00055)	7170..7691	+	522	WP_000198608.1	hypothetical protein	-
L1971_RS00060 (L1971_00060)	7726..8508	+	783	WP_000082169.1	site-specific integrase	-
L1971_RS00065 (L1971_00065)	8517..9230	+	714	WP_237123644.1	EAL domain-containing protein	-
L1971_RS00070 (L1971_00070)	9255..9743	+	489	WP_237123648.1	CaiF/GrlA family transcriptional regulator	-
L1971_RS00075 (L1971_00075)	9737..10222	+	486	WP_000905606.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	fdeC / spvC / spvB / rck / pefD / pefC / pefA / pefB	1..104427	104427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15139.68 Da
• Isoelectric Point: 8.5258

>T233077 WP_001697179.1 NZ_CP091299:c5385-4969 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
VKKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAVLPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T233077 NZ_CP128526:c2268964-2268695 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8797.94 Da
• Isoelectric Point: 4.3775

>AT233077 WP_001261289.1 NZ_CP091299:c5612-5382 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT233077 NZ_CP128526:c2269217-2268951 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA