Detailed information of TA system TA233076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 21618..22219
• Replicon: plasmid p1186-2
• Accession: NZ_CP091296
• Organism: Lactiplantibacillus plantarum subsp. plantarum strain GR1186

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: L3C66_RS15465
• Protein ID: WP_001748110.1
• Coordinates: 21618..21962 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: L3C66_RS15470
• Protein ID: WP_001748109.1
• Coordinates: 21956..22219 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3C66_RS15430 (L3C66_15430)	17007..17414	+	408	WP_046811104.1	hypothetical protein	-
L3C66_RS15435 (L3C66_15435)	17530..17940	+	411	WP_046811105.1	hypothetical protein	-
L3C66_RS15440 (L3C66_15440)	18063..18365	-	303	WP_005917927.1	type II toxin-antitoxin system YafQ family toxin	-
L3C66_RS15445 (L3C66_15445)	18355..18633	-	279	WP_003625288.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
L3C66_RS15450 (L3C66_15450)	18997..19185	-	189	Protein_21	IS3 family transposase	-
L3C66_RS15455 (L3C66_15455)	19311..20363	+	1053	WP_046811107.1	ABC transporter permease	-
L3C66_RS15460 (L3C66_15460)	20368..21048	+	681	WP_046811108.1	ABC transporter ATP-binding protein	-
L3C66_RS15465 (L3C66_15465)	21618..21962	-	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L3C66_RS15470 (L3C66_15470)	21956..22219	-	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L3C66_RS15475 (L3C66_15475)	22305..22892	+	588	WP_046811109.1	site-specific integrase	-
L3C66_RS15480 (L3C66_15480)	23266..23460	+	195	WP_046811110.1	hypothetical protein	-
L3C66_RS15485 (L3C66_15485)	23707..24491	+	785	WP_225351411.1	IS5 family transposase	-
L3C66_RS15490 (L3C66_15490)	25353..25730	-	378	WP_052748124.1	YxeA family protein	-
L3C66_RS15495 (L3C66_15495)	26444..26947	-	504	WP_046811094.1	DUF536 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..31995	31995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T233076 WP_001748110.1 NZ_CP091296:c21962-21618 [Lactiplantibacillus plantarum subsp. plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T233076 NZ_CP128526:1758483-1758707 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT233076 WP_001748109.1 NZ_CP091296:c22219-21956 [Lactiplantibacillus plantarum subsp. plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT233076 NZ_CP128526:1758750-1759079 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A806J926