Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2653887..2654538 Replicon chromosome
Accession NZ_CP091289
Organism Lacticaseibacillus paracasei subsp. paracasei strain GR0562

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag L3C06_RS12865 Protein ID WP_003567661.1
Coordinates 2653887..2654270 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag L3C06_RS12870 Protein ID WP_003567665.1
Coordinates 2654290..2654538 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3C06_RS12855 (L3C06_12855) 2651712..2652233 - 522 WP_003567657.1 QueT transporter family protein -
L3C06_RS12860 (L3C06_12860) 2652444..2653688 + 1245 WP_077069322.1 ISL3 family transposase -
L3C06_RS12865 (L3C06_12865) 2653887..2654270 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L3C06_RS12870 (L3C06_12870) 2654290..2654538 - 249 WP_003567665.1 antitoxin Antitoxin
L3C06_RS12875 (L3C06_12875) 2654606..2655742 - 1137 WP_016369534.1 alanine racemase -
L3C06_RS12880 (L3C06_12880) 2655729..2656103 - 375 WP_003567669.1 holo-ACP synthase -
L3C06_RS12885 (L3C06_12885) 2656273..2657781 - 1509 WP_016387847.1 DEAD/DEAH box helicase -
L3C06_RS12890 (L3C06_12890) 2657907..2658050 - 144 WP_003588978.1 hypothetical protein -
L3C06_RS12895 (L3C06_12895) 2658081..2659469 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T233075 WP_003567661.1 NZ_CP091289:c2654270-2653887 [Lacticaseibacillus paracasei subsp. paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T233075 NZ_CP128526:1736342-1736611 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT233075 WP_003567665.1 NZ_CP091289:c2654538-2654290 [Lacticaseibacillus paracasei subsp. paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT233075 NZ_CP128526:1736072-1736338 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References