Detailed information of TA system TA233073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 447080..447708
• Replicon: chromosome
• Accession: NZ_CP091289
• Organism: Lacticaseibacillus paracasei subsp. paracasei strain GR0562

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S2NVS0
• Locus tag: L3C06_RS02020
• Protein ID: WP_016373562.1
• Coordinates: 447340..447708 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: L3C06_RS02015
• Protein ID: WP_003583264.1
• Coordinates: 447080..447340 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3C06_RS01985 (L3C06_01985)	442870..443400	+	531	WP_012490988.1	DNA-directed RNA polymerase subunit sigma-24	-
L3C06_RS01990 (L3C06_01990)	443568..443720	+	153	WP_003563363.1	YvrJ family protein	-
L3C06_RS01995 (L3C06_01995)	443730..444494	+	765	WP_016387929.1	GH25 family lysozyme	-
L3C06_RS02000 (L3C06_02000)	444705..445208	+	504	WP_003563372.1	TetR/AcrR family transcriptional regulator	-
L3C06_RS02005 (L3C06_02005)	445213..446274	+	1062	WP_016387927.1	ABC transporter permease	-
L3C06_RS02010 (L3C06_02010)	446279..446968	+	690	WP_016387926.1	ABC transporter ATP-binding protein	-
L3C06_RS02015 (L3C06_02015)	447080..447340	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
L3C06_RS02020 (L3C06_02020)	447340..447708	-	369	WP_016373562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3C06_RS02025 (L3C06_02025)	448000..449370	-	1371	WP_016387925.1	FAD-dependent oxidoreductase	-
L3C06_RS02030 (L3C06_02030)	449734..450552	-	819	WP_019914724.1	Cof-type HAD-IIB family hydrolase	-
L3C06_RS02035 (L3C06_02035)	450869..451138	-	270	WP_003563382.1	type II toxin-antitoxin system YafQ family toxin	-
L3C06_RS02040 (L3C06_02040)	451379..452386	+	1008	WP_236360577.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14923.01 Da
• Isoelectric Point: 8.3986

>T233073 WP_016373562.1 NZ_CP091289:c447708-447340 [Lacticaseibacillus paracasei subsp. paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMPERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQANLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T233073 NZ_CP128526:c945039-944857 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT233073 WP_003583264.1 NZ_CP091289:c447340-447080 [Lacticaseibacillus paracasei subsp. paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT233073 NZ_CP128526:c944818-944399 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: S2NVS0