Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1966729..1967220 Replicon chromosome
Accession NZ_CP091281
Organism Macrococcus canis strain B8

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag L2Z53_RS10325 Protein ID WP_086043355.1
Coordinates 1966729..1967079 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag L2Z53_RS10330 Protein ID WP_101495293.1
Coordinates 1967080..1967220 (-) Length 47 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L2Z53_RS10300 (L2Z53_10300) 1961837..1963972 - 2136 WP_236365975.1 Tex family protein -
L2Z53_RS10305 (L2Z53_10305) 1964049..1964816 - 768 WP_133420462.1 RNA polymerase sigma factor SigB -
L2Z53_RS10310 (L2Z53_10310) 1964821..1965264 - 444 WP_086043352.1 anti-sigma B factor RsbW -
L2Z53_RS10315 (L2Z53_10315) 1965269..1965592 - 324 WP_086043353.1 anti-sigma factor antagonist -
L2Z53_RS10320 (L2Z53_10320) 1965673..1966680 - 1008 WP_086043354.1 PP2C family protein-serine/threonine phosphatase -
L2Z53_RS10325 (L2Z53_10325) 1966729..1967079 - 351 WP_086043355.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L2Z53_RS10330 (L2Z53_10330) 1967080..1967220 - 141 WP_101495293.1 antitoxin MazE Antitoxin
L2Z53_RS10335 (L2Z53_10335) 1967282..1968424 - 1143 WP_236365976.1 alanine racemase -
L2Z53_RS10340 (L2Z53_10340) 1968450..1968806 - 357 WP_086043357.1 holo-ACP synthase -
L2Z53_RS10345 (L2Z53_10345) 1968748..1969269 - 522 WP_236365977.1 PH domain-containing protein -
L2Z53_RS10350 (L2Z53_10350) 1969253..1970764 - 1512 WP_236365978.1 PH domain-containing protein -
L2Z53_RS10355 (L2Z53_10355) 1970754..1971230 - 477 WP_086043360.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12705.88 Da        Isoelectric Point: 9.9423

>T233070 WP_086043355.1 NZ_CP091281:c1967079-1966729 [Macrococcus canis]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNNIGNKHSPTVIVAAITAKINKAKIPTHVEIGKTTHHLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDVALLISLGLSIDSE

Download         Length: 351 bp

>T233070 NZ_CP128526:c25018-24590 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin


Download         Length: 47 a.a.        Molecular weight: 5319.89 Da        Isoelectric Point: 3.8301

>AT233070 WP_101495293.1 NZ_CP091281:c1967220-1967080 [Macrococcus canis]
MSQKLEQMLIEGYQSMAEINLSIASEHYILECEVNDNHQTYTLDGE

Download         Length: 141 bp

>AT233070 NZ_CP128526:c25305-25018 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References