Detailed information of TA system TA233035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2720376..2720947
• Replicon: chromosome
• Accession: NZ_CP091240
• Organism: Enterococcus faecalis strain 249

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LQ065_RS13055
• Protein ID: WP_002354774.1
• Coordinates: 2720376..2720717 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: LQ065_RS13060
• Protein ID: WP_002354773.1
• Coordinates: 2720717..2720947 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ065_RS13050 (2716391)	2716391..2720005	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
LQ065_RS13055 (2720376)	2720376..2720717	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LQ065_RS13060 (2720717)	2720717..2720947	-	231	WP_002354773.1	hypothetical protein	Antitoxin
LQ065_RS13065 (2721354)	2721354..2721569	-	216	WP_002358974.1	zinc ribbon domain-containing protein	-
LQ065_RS13070 (2721708)	2721708..2722700	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
LQ065_RS13075 (2722767)	2722767..2723399	-	633	WP_002358972.1	RloB family protein	-
LQ065_RS13080 (2723408)	2723408..2724703	-	1296	WP_002396787.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T233035 WP_002354774.1 NZ_CP091240:c2720717-2720376 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T233035 NZ_CP128520:c769799-769530 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT233035 WP_002354773.1 NZ_CP091240:c2720947-2720717 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT233035 NZ_CP128520:c770069-769803 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812