Detailed information of TA system TA233026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 62067..63204
• Replicon: plasmid p1521-a
• Accession: NZ_CP091238
• Organism: Enterococcus faecalis strain 1521

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: P0A4M2
• Locus tag: LQ062_RS13045
• Protein ID: WP_002332783.1
• Coordinates: 62067..62930 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL19
• Locus tag: LQ062_RS13050
• Protein ID: WP_002326825.1
• Coordinates: 62932..63204 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ062_RS12995 (LQ062_12995)	57217..57837	+	621	WP_002367784.1	recombinase family protein	-
LQ062_RS13000 (LQ062_13000)	57827..58141	+	315	WP_002367785.1	hypothetical protein	-
LQ062_RS13005 (LQ062_13005)	58135..58341	+	207	WP_002367786.1	hypothetical protein	-
LQ062_RS13010 (LQ062_13010)	58501..58695	+	195	WP_002367787.1	hypothetical protein	-
LQ062_RS13015 (LQ062_13015)	58707..58898	+	192	WP_002367788.1	hypothetical protein	-
LQ062_RS13020 (LQ062_13020)	59068..59283	+	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
LQ062_RS13025 (LQ062_13025)	59284..59625	+	342	WP_002367792.1	bacteriocin immunity protein	-
LQ062_RS13030 (LQ062_13030)	59793..60473	+	681	WP_010710189.1	IS6-like element IS1216 family transposase	-
LQ062_RS13035 (LQ062_13035)	60507..61013	-	507	WP_002415429.1	trimethoprim-resistant dihydrofolate reductase DfrG	-
LQ062_RS13040 (LQ062_13040)	61310..61627	-	318	WP_002326830.1	hypothetical protein	-
LQ062_RS13045 (LQ062_13045)	62067..62930	-	864	WP_002332783.1	zeta toxin family protein	Toxin
LQ062_RS13050 (LQ062_13050)	62932..63204	-	273	WP_002326825.1	antitoxin	Antitoxin
LQ062_RS13055 (LQ062_13055)	63222..63437	-	216	WP_001835296.1	peptide-binding protein	-
LQ062_RS13060 (LQ062_13060)	63529..64425	-	897	WP_002326827.1	ParA family protein	-
LQ062_RS13065 (LQ062_13065)	64528..64788	-	261	Protein_76	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
LQ062_RS13070 (LQ062_13070)	64958..65695	-	738	WP_001038795.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
LQ062_RS13075 (LQ062_13075)	65820..65903	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
LQ062_RS13160	65952..66035	-	84	Protein_79	MLS leader peptide	-
LQ062_RS13080 (LQ062_13080)	66335..66706	-	372	WP_002358205.1	hypothetical protein	-
LQ062_RS13085 (LQ062_13085)	66699..67544	-	846	WP_000239313.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cat / tet(L) / tet(M) / dfrG / erm(B)	-	1..68015	68015
	-	inside	IS/Tn	dfrG / erm(B)	-	59787..65695	5908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32675.27 Da
• Isoelectric Point: 7.3939

>T233026 WP_002332783.1 NZ_CP091238:c62930-62067 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T233026 NZ_CP128519:1324582-1324758 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10720.17 Da
• Isoelectric Point: 4.4865

>AT233026 WP_002326825.1 NZ_CP091238:c63204-62932 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Nucleotide

• Length: 273 bp

>AT233026 NZ_CP128519:1324814-1325230 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: P0A4M2

Antitoxin

• AlphaFold DB: A0A0M2AF93