Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907(toxin) |
| Location | 56442..57013 | Replicon | plasmid p1521-a |
| Accession | NZ_CP091238 | ||
| Organism | Enterococcus faecalis strain 1521 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | A0A2Z2CGM4 |
| Locus tag | LQ062_RS12985 | Protein ID | WP_002362432.1 |
| Coordinates | 56442..56783 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | R3KHK9 |
| Locus tag | LQ062_RS12990 | Protein ID | WP_002362431.1 |
| Coordinates | 56783..57013 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ062_RS12960 (LQ062_12960) | 51459..52151 | - | 693 | WP_002415124.1 | Fic family protein | - |
| LQ062_RS12965 (LQ062_12965) | 52200..52799 | - | 600 | WP_002415125.1 | tyrosine-type recombinase/integrase | - |
| LQ062_RS12975 (LQ062_12975) | 54525..55127 | - | 603 | WP_002362434.1 | Fic family protein | - |
| LQ062_RS12980 (LQ062_12980) | 55392..56330 | - | 939 | WP_002362433.1 | hypothetical protein | - |
| LQ062_RS12985 (LQ062_12985) | 56442..56783 | - | 342 | WP_002362432.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LQ062_RS12990 (LQ062_12990) | 56783..57013 | - | 231 | WP_002362431.1 | hypothetical protein | Antitoxin |
| LQ062_RS12995 (LQ062_12995) | 57217..57837 | + | 621 | WP_002367784.1 | recombinase family protein | - |
| LQ062_RS13000 (LQ062_13000) | 57827..58141 | + | 315 | WP_002367785.1 | hypothetical protein | - |
| LQ062_RS13005 (LQ062_13005) | 58135..58341 | + | 207 | WP_002367786.1 | hypothetical protein | - |
| LQ062_RS13010 (LQ062_13010) | 58501..58695 | + | 195 | WP_002367787.1 | hypothetical protein | - |
| LQ062_RS13015 (LQ062_13015) | 58707..58898 | + | 192 | WP_002367788.1 | hypothetical protein | - |
| LQ062_RS13020 (LQ062_13020) | 59068..59283 | + | 216 | WP_002367791.1 | leucocin A/sakacin P family class II bacteriocin | - |
| LQ062_RS13025 (LQ062_13025) | 59284..59625 | + | 342 | WP_002367792.1 | bacteriocin immunity protein | - |
| LQ062_RS13030 (LQ062_13030) | 59793..60473 | + | 681 | WP_010710189.1 | IS6-like element IS1216 family transposase | - |
| LQ062_RS13035 (LQ062_13035) | 60507..61013 | - | 507 | WP_002415429.1 | trimethoprim-resistant dihydrofolate reductase DfrG | - |
| LQ062_RS13040 (LQ062_13040) | 61310..61627 | - | 318 | WP_002326830.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | cat / tet(L) / tet(M) / dfrG / erm(B) | - | 1..68015 | 68015 | |
| - | inside | IS/Tn | dfrG / erm(B) | - | 59787..65695 | 5908 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13244.44 Da Isoelectric Point: 8.0113
>T233025 WP_002362432.1 NZ_CP091238:c56783-56442 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T233025 NZ_CP128519:c954953-954771 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8510.42 Da Isoelectric Point: 4.3855
>AT233025 WP_002362431.1 NZ_CP091238:c57013-56783 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
Download Length: 231 bp
>AT233025 NZ_CP128519:c954732-954313 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z2CGM4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R3KHK9 |